User:Helen L. Slucher/Notebook/CHEM 571/2013/11/05: Difference between revisions

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==Procedure==
# Lysozyme AuNP and Myoglobin AuNP
## Determine the total amounts of materials you will need for the following samples using the same procedures as the last two times we made protein-AuNPs (final Au concentration of 0.25mM and a final volume of 5mL)
### Lysozyme
#### 30
#### 50
#### 70
#### 90
#### 110
#### 130
#### 150
#### 170
#### 190
#### 210
### Myoglobin
#### 10
#### 15
#### 25
#### 30
#### 35
#### 40
#### 45
#### 50
## Make your stock solutions
## Make your samples in small test tubes and cap with aluminum foil
## Heat your samples at 80C for 4 hours


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Revision as of 17:03, 2 November 2013

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Procedure

  1. Lysozyme AuNP and Myoglobin AuNP
    1. Determine the total amounts of materials you will need for the following samples using the same procedures as the last two times we made protein-AuNPs (final Au concentration of 0.25mM and a final volume of 5mL)
      1. Lysozyme
        1. 30
        2. 50
        3. 70
        4. 90
        5. 110
        6. 130
        7. 150
        8. 170
        9. 190
        10. 210
      2. Myoglobin
        1. 10
        2. 15
        3. 25
        4. 30
        5. 35
        6. 40
        7. 45
        8. 50
    2. Make your stock solutions
    3. Make your samples in small test tubes and cap with aluminum foil
    4. Heat your samples at 80C for 4 hours