User:Helen L. Slucher/Notebook/CHEM 571/2013/11/13

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(UV-Vis)
 
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# Centrifuge 1mL of the sample for 30 min at max rpm.
# Centrifuge 1mL of the sample for 30 min at max rpm.
# Take UV-Vis measurements of the samples.
# Take UV-Vis measurements of the samples.
-
 
Solutions with the following Au:protein ratio were run on the UV-Vis:
Solutions with the following Au:protein ratio were run on the UV-Vis:
:''Gold to Lysozyme Ratio''
:''Gold to Lysozyme Ratio''
Line 37: Line 36:
 +
*Make the following solutions:
 +
:''Gold to Hemoglobin Ratio''
 +
::*30
 +
::*50
 +
::*70
 +
::*90
 +
::*110
 +
::*130
 +
::*150
 +
::*170
 +
::*190
 +
::*210
 +
:''Gold to Lysozyme Ratio''
 +
::*5
 +
::*10
 +
::*15
 +
::*20
 +
::*25
 +
::*30
 +
::*35
 +
::*40
 +
::*45
 +
::*50
 +
 +
==Data==
 +
*[[Image:Screen Shot 2013-12-11 at 4.27.00 PM.png]]
 +
 +
==UV-Vis==
 +
*[[Image:Screen Shot 2013-12-11 at 6.19.31 PM.png]]
 +
*Series 1-10
 +
::*30
 +
::*50
 +
::*70
 +
::*90
 +
::*110
 +
::*130
 +
::*150
 +
::*170
 +
::*190
 +
::*210
 +
*[[Image:Screen Shot 2013-12-11 at 6.19.48 PM.png]]
 +
*Series 1-10
 +
::*5
 +
::*10
 +
::*15
 +
::*20
 +
::*25
 +
::*30
 +
::*35
 +
::*40
 +
::*45
 +
::*50
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__NOTOC__
__NOTOC__

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11/12/2013

Procedure

  1. Centrifuge 1mL of the sample for 30 min at max rpm.
  2. Take UV-Vis measurements of the samples.

Solutions with the following Au:protein ratio were run on the UV-Vis:

Gold to Lysozyme Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Myoglobin Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50


  • Make the following solutions:
Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50

Data

  • Image:Screen Shot 2013-12-11 at 4.27.00 PM.png

UV-Vis

  • Image:Screen Shot 2013-12-11 at 6.19.31 PM.png
  • Series 1-10
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
  • Image:Screen Shot 2013-12-11 at 6.19.48 PM.png
  • Series 1-10
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50


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