User:Helen L. Slucher/Notebook/CHEM 571/2013/11/13: Difference between revisions

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# Centrifuge 1mL of the sample for 30 min at max rpm.
# Centrifuge 1mL of the sample for 30 min at max rpm.
# Take UV-Vis measurements of the samples.
# Take UV-Vis measurements of the samples.
Solutions with the following Au:protein ratio were run on the UV-Vis:
Solutions with the following Au:protein ratio were run on the UV-Vis:
:''Gold to Lysozyme Ratio''
:''Gold to Lysozyme Ratio''
Line 37: Line 36:




*Make the following solutions:
:''Gold to Hemoglobin Ratio''
::*30
::*50
::*70
::*90
::*110
::*130
::*150
::*170
::*190
::*210
:''Gold to Lysozyme Ratio''
::*5
::*10
::*15
::*20
::*25
::*30
::*35
::*40
::*45
::*50
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__NOTOC__
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Revision as of 14:26, 11 December 2013

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11/12/2013

Procedure

  1. Centrifuge 1mL of the sample for 30 min at max rpm.
  2. Take UV-Vis measurements of the samples.

Solutions with the following Au:protein ratio were run on the UV-Vis:

Gold to Lysozyme Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Myoglobin Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50


  • Make the following solutions:
Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50