# User:Helen L. Slucher/Notebook/CHEM 571/2013/11/13

(Difference between revisions)
 Revision as of 17:15, 11 December 2013 (view source) (→Entry title)← Previous diff Revision as of 17:26, 11 December 2013 (view source) (→Procedure)Next diff → Line 11: Line 11: # Centrifuge 1mL of the sample for 30 min at max rpm. # Centrifuge 1mL of the sample for 30 min at max rpm. # Take UV-Vis measurements of the samples. # Take UV-Vis measurements of the samples. - Solutions with the following Au:protein ratio were run on the UV-Vis: Solutions with the following Au:protein ratio were run on the UV-Vis: :''Gold to Lysozyme Ratio'' :''Gold to Lysozyme Ratio'' Line 37: Line 36: + *Make the following solutions: + :''Gold to Hemoglobin Ratio'' + ::*30 + ::*50 + ::*70 + ::*90 + ::*110 + ::*130 + ::*150 + ::*170 + ::*190 + ::*210 + :''Gold to Lysozyme Ratio'' + ::*5 + ::*10 + ::*15 + ::*20 + ::*25 + ::*30 + ::*35 + ::*40 + ::*45 + ::*50 |} |} __NOTOC__ __NOTOC__

## Revision as of 17:26, 11 December 2013

Project name Main project page
Previous entry      Next entry

## Procedure

1. Centrifuge 1mL of the sample for 30 min at max rpm.
2. Take UV-Vis measurements of the samples.

Solutions with the following Au:protein ratio were run on the UV-Vis:

Gold to Lysozyme Ratio
• 30
• 50
• 70
• 90
• 110
• 130
• 150
• 170
• 190
• 210
Gold to Myoglobin Ratio
• 5
• 10
• 15
• 20
• 25
• 30
• 35
• 40
• 45
• 50

• Make the following solutions:
Gold to Hemoglobin Ratio
• 30
• 50
• 70
• 90
• 110
• 130
• 150
• 170
• 190
• 210
Gold to Lysozyme Ratio
• 5
• 10
• 15
• 20
• 25
• 30
• 35
• 40
• 45
• 50