User:Hetmann/Notebook/2006-11-22: Difference between revisions
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Ligation 3:1 by volume, plates (3) are in the 37 - take out by 11am Thanksgiving. | Ligation 3:1 by volume, plates (3) are in the 37 - take out by 11am Thanksgiving. | ||
==Notes== | |||
The last time this step was attempted, it failed as well - we might have a problem with the gel purification step (maybe needs to be debugged, or new reagents). | |||
Latest revision as of 11:10, 23 November 2006
Peng's Notes
did gel purification, but extremely low numbers (less than 10) leads me to believe it didnt work.
Ligation 3:1 by volume, plates (3) are in the 37 - take out by 11am Thanksgiving.
Notes
The last time this step was attempted, it failed as well - we might have a problem with the gel purification step (maybe needs to be debugged, or new reagents).
