User:Hetmann/Notebook/2006-6-12: Difference between revisions

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'''First summer morning meeting '''
= Official Start Day =


Discussion of potential topics, a potential team name.
== Start of Week 1 ==
[[http://openwetware.org/wiki/User:Zhipeng_Sun#June_12th Zhipeng's Notes on the Meeting]]
 
==== First Monday Morning Meeting ====
 
*Discussion of potential topics, a potential team name.
*Team name suggestion from Perry: '''[http://planetcybertron.com/wallpaper-g1_800x600-1.jpg Transformers]'''
*[[http://openwetware.org/wiki/User:Zhipeng_Sun#June_12th Zhipeng's Notes on the Meeting]]


Profs. George Church, Pam Silver, Alain Viel; TFs Chris Douchette, Shawn Douglas, Radhika Nagpal
Profs. George Church, Pam Silver, Alain Viel; TFs Chris Douchette, Shawn Douglas, Radhika Nagpal


'''Nanostructure folding'''
==== Day Overview ====
 
==== Nanostructure folding ====
 
Followed [[IGEM:Harvard/2006/Folding_DNA_nanostructures| Shawn's Protocol]] to fold nanostructures.
 
Reactions were mixed and placed into tubes as labeled as follows:
 
*'''HH JL''' (representing oligonucleotides + scaffold)
*'''HH JL -o''' (representing a lack of oligonucleotides)
*'''HH JL -s''' (representing a lack of scaffolds)
 
==== Transformation ====


Worked through [[IGEM:Harvard/2006/Folding_DNA_nanostructures| Shawn's Protocol]] to fold nanostructures.
Transformed the following BioBrick plasmids into OneShot Top10 competent cells (Invitrogen)


Reactions were mixed and placed into labeled tubes.
*R0010
*E7104 
*E0241

Revision as of 12:28, 29 June 2006

Official Start Day

Start of Week 1

First Monday Morning Meeting

Profs. George Church, Pam Silver, Alain Viel; TFs Chris Douchette, Shawn Douglas, Radhika Nagpal

Day Overview

Nanostructure folding

Followed Shawn's Protocol to fold nanostructures.

Reactions were mixed and placed into tubes as labeled as follows:

  • HH JL (representing oligonucleotides + scaffold)
  • HH JL -o (representing a lack of oligonucleotides)
  • HH JL -s (representing a lack of scaffolds)

Transformation

Transformed the following BioBrick plasmids into OneShot Top10 competent cells (Invitrogen)

  • R0010
  • E7104
  • E0241