User:Hetmann/Notebook/2006-6-12: Difference between revisions
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= Official Start Day = | |||
Discussion of potential topics, a potential team name. | == Start of Week 1 == | ||
[[http://openwetware.org/wiki/User:Zhipeng_Sun#June_12th Zhipeng's Notes on the Meeting]] | |||
==== First Monday Morning Meeting ==== | |||
*Discussion of potential topics, a potential team name. | |||
*Team name suggestion from Perry: '''[http://planetcybertron.com/wallpaper-g1_800x600-1.jpg Transformers]''' | |||
*[[http://openwetware.org/wiki/User:Zhipeng_Sun#June_12th Zhipeng's Notes on the Meeting]] | |||
Profs. George Church, Pam Silver, Alain Viel; TFs Chris Douchette, Shawn Douglas, Radhika Nagpal | Profs. George Church, Pam Silver, Alain Viel; TFs Chris Douchette, Shawn Douglas, Radhika Nagpal | ||
''' | ==== Day Overview ==== | ||
==== Nanostructure folding ==== | |||
Followed [[IGEM:Harvard/2006/Folding_DNA_nanostructures| Shawn's Protocol]] to fold nanostructures. | |||
Reactions were mixed and placed into tubes as labeled as follows: | |||
*'''HH JL''' (representing oligonucleotides + scaffold) | |||
*'''HH JL -o''' (representing a lack of oligonucleotides) | |||
*'''HH JL -s''' (representing a lack of scaffolds) | |||
==== Transformation ==== | |||
Transformed the following BioBrick plasmids into OneShot Top10 competent cells (Invitrogen) | |||
*R0010 | |||
*E7104 | |||
*E0241 |
Revision as of 12:28, 29 June 2006
Official Start Day
Start of Week 1
First Monday Morning Meeting
- Discussion of potential topics, a potential team name.
- Team name suggestion from Perry: Transformers
- [Zhipeng's Notes on the Meeting]
Profs. George Church, Pam Silver, Alain Viel; TFs Chris Douchette, Shawn Douglas, Radhika Nagpal
Day Overview
Nanostructure folding
Followed Shawn's Protocol to fold nanostructures.
Reactions were mixed and placed into tubes as labeled as follows:
- HH JL (representing oligonucleotides + scaffold)
- HH JL -o (representing a lack of oligonucleotides)
- HH JL -s (representing a lack of scaffolds)
Transformation
Transformed the following BioBrick plasmids into OneShot Top10 competent cells (Invitrogen)
- R0010
- E7104
- E0241