User:Hetmann/Notebook/2007-3-21

Hello,

It's been a long time since I've been in lab, though that should change in the upcoming weeks. I'll try to finish about 5 westerns by the end of the semester, and depending on the amount of time that I have, I may also work into reading period (after my 91r is due).

Next up: plans for a second experimental Western blot.

Key differences:

• Inclusion of a different primary antibody that detects levels of a protein that is consistently produced in E. coli
  • This antibody is limited to the following bio-tech companies:
    • Invitrogen
    • Sigma
    • Santa Cruz
  • A good place to start is here
• Instead of taking time points every half-hour, instead we are going to be taking time points once every 3 hours.
  • Justification:
    1. It will be less time consuming to collect data points over the span of 1 day
    2. The data will fit on the gel much easier (allowing for two positive controls: J36834 (KaiC + promoter) and J36335 (KaiA, KaiC + promoter)

• Possible protein to be used.