User:Hetmann/Notebook/2007-3-21

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: Hello, It's been a long time since I've been in lab, though that should change in the upcoming weeks. I'll try to finish about 5 westerns by the end of the semester, and depending on the...)
Line 17: Line 17:
:**# It will be less time consuming to collect data points over the span of 1 day
:**# It will be less time consuming to collect data points over the span of 1 day
:**# The data will fit on the gel much easier (allowing for two positive controls: J36834 (KaiC + promoter) and J36335 (KaiA, KaiC + promoter)
:**# The data will fit on the gel much easier (allowing for two positive controls: J36834 (KaiC + promoter) and J36335 (KaiA, KaiC + promoter)
 +
 +
:*Possible [http://www.biocompare.com/itemdetails.asp?itemid=311763 protein] to be used.

Revision as of 14:02, 21 March 2007

Hello,

It's been a long time since I've been in lab, though that should change in the upcoming weeks. I'll try to finish about 5 westerns by the end of the semester, and depending on the amount of time that I have, I may also work into reading period (after my 91r is due).

Next up: plans for a second experimental Western blot.

Key differences:

  • Inclusion of a different primary antibody that detects levels of a protein that is consistently produced in E. coli
    • This antibody is limited to the following bio-tech companies:
      • Invitrogen
      • Sigma
      • Santa Cruz
    • A good place to start is here
  • Instead of taking time points every half-hour, instead we are going to be taking time points once every 3 hours.
    • Justification:
      1. It will be less time consuming to collect data points over the span of 1 day
      2. The data will fit on the gel much easier (allowing for two positive controls: J36834 (KaiC + promoter) and J36335 (KaiA, KaiC + promoter)
Personal tools