User:Howard Boland/Notebook/Art from Synthetic Biology/2010/08/11
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Double digestion pUA66 XhoI / BamHITwo new double digestion was set up to run overnight. For a final volume of 50µl
Tube 1 was placed in the PCR machine at 37ºC. Tube 2 was placed in the incubator at 37ºC.
Gel Purification of digests from 09 of August 2010This purification follows the digestion performed User:Howard Boland/Notebook/Art from Synthetic Biology/2010/08/09. All 6 digestions from the pUA66 where purified by performing a cutting from the gel and then purifying the gel band.
The procedure was carried out in ration relations to the band weight. e.g. 300mg => 300µl in 1:1 and 300mg => 900µl in 1:3
Gel verificationIn order to check that the purified vectors retain the band and strength a gel was set up as follows
Gel Result: Note: If the purified DNA is to be analyzed on a gel, add 1 volume of Loading Dye to 5 volumes of purified DNA. Mix the solution by pipetting up and down before loading the gel. |