User:Howard Boland/Notebook/Art from Synthetic Biology/2010/10/26
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Gel pUA66katE template, product pSense66 - 2359bpShould have used 1% Gel, 1kb ladder - product loaded on wrong gel (gel mix-up)
Gel pMAK512 template, product pBR322 - 790bpShould have used 2% Gel, 100bp ladder - product loaded on wrong gel (gel mix-up)
PCR OptimisationThis protocol is a review to correct the PCR conditions from Friday. Mastermix PCR - pMAK512
Cycles: 30x Lid: 100ºC Volume: 50µl (each)
Cycles: 30x Lid: 100ºC Volume: 50µl (each)
Possible error: There was a lot of kerfuffle around me whilst setting up these reactions Gel purificationThe last step I did was to purify products from the gels. Gels were however not accurate and I will need to check these purified products tomorrow on correct gels. I purified pBR322 from fridays PCR and digestion (overnight and 2 hour) and one of the pSense66 products from the overnight digestion. |