User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/09
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Transformation Check BackbonesAs expected the chloramphenicol plate was doggy, the other plates grew fine
Overnight Growth LigationThe ligation grew fine but this is expected to not have worked so part J04550 remains - colour is red/pink Miniprep LigationI extracted the plasmid of the proposed ligation growth above Overnight Growth strainsAll strains grew:
Competent CellsI prepared 3 strains of competent cells:
Check Competent CellsI set up 3 transformations with J04450, Plate 1, A1, Spring 2010.
Transformation pSB1C3I set up a new transformation of this part as the previous chloramphenicol plate was doggy Overnight Growth BioBrick Backbones10ml LB-Broth with respective antibiotics
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