User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/03/17: Difference between revisions
From OpenWetWare
(Autocreate 2010/03/17 Entry for User:Igor_R._Kuznetsov/Notebook/RBL-2H3) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==U937== | ||
Cells suspended in RPMI 1640, <math>1x10^7 cells/mL</math>. Coverslips coated with Poly-L-lysine (P4832 SIGMA) for 30 minutes, washed 2 times with phosphate saline buffer buffer. Plated media with cells for 30 minutes, then washed with phosphate saline buffer two times to remove suspended cells. | |||
'''Sample 1:''' phosphate saline buffer, left at room temperature for 30 minutes | |||
[[Image:U937_01.jpg]] | |||
'''Sample 2:''' N-fMLFF <math>10^{-7} \mu M</math>, in phosphate saline buffer, left at room temperature for 30 minutes | |||
[[Image:U937_02.jpg]] | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 19:00, 17 March 2010
 RBL-2H3
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
U937Cells suspended in RPMI 1640, [math]\displaystyle{ 1x10^7 cells/mL }[/math]. Coverslips coated with Poly-L-lysine (P4832 SIGMA) for 30 minutes, washed 2 times with phosphate saline buffer buffer. Plated media with cells for 30 minutes, then washed with phosphate saline buffer two times to remove suspended cells. Sample 1: phosphate saline buffer, left at room temperature for 30 minutes
Sample 2: N-fMLFF [math]\displaystyle{ 10^{-7} \mu M }[/math], in phosphate saline buffer, left at room temperature for 30 minutes
|
|
