User:Ilya/Yeast/Mating pheromone response pathway

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  • Crosstalk in the pathways is eliminated by formation of pathway-specific complexes (Ste5:Ste11:Ste7:Fus3, Pbs2:Ste11:Hog1)
  • In S. cerevisiae, meiosis is initiated only by diploid cells deprived of glucose and nitrogen and grown in the presence of a nonfermentable carbon source [1]

Pheromone processing genes

  • STE14
    • carries out C-terminal methylation of prenylated proteins including a-factor, Ras1p, and Ras2p
    • encodes the prenylcysteine-dependent carboxyl methyltransferase that mediates methylation, the final step in modification of CAAX proteins; Ste14p is also membrane associated [2]
  • STE13
    • Dipeptidyl aminopeptidase A involved in maturation of a-factor
  • AXL1
    • Protease involved in proteolytic step of a-factor N-terminal processing
  • STE23
  • RAM1, RAM2
    • Required for a-factor prenylation
  • STE24
  • RCE1
  • KEX2
  • KEX1
  • STE23

Other genes

These genes are part of the mating machinery in yeast but not necessary part of the mating pheromone response pathway.

  • Swe1 - protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity
  • Ras1 - pombe homolog interacts with MAPKKK
  • SAG1 (α-agglutinin) - provides tight cell-cell adhesion during mating in S. cerevisiae.
  • FUS1 - protein with SH3 domain required for cell fusion during mating, located at the tip of the mating projection
  • BNI1/YNL271C (Saccharomyces cerevisiae)
    • Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1


  • Pathway architecture overview
  1. Tzung KW, Williams RM, Scherer S, Federspiel N, Jones T, Hansen N, Bivolarevic V, Huizar L, Komp C, Surzycki R, Tamse R, Davis RW, and Agabian N. . pmid:11248064. PubMed HubMed [5]
  2. Ashby MN, Errada PR, Boyartchuk VL, and Rine J. . pmid:8212897. PubMed HubMed [4]
  3. Kofahl B and Klipp E. . pmid:15300679. PubMed HubMed [1]
  4. Banuett F. . pmid:9618441. PubMed HubMed [2]
  5. Bardwell L. . pmid:15374648. PubMed HubMed [3]
All Medline abstracts: PubMed HubMed
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