User:Ilya/Yeast/Mating pheromone response pathway: Difference between revisions
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*[[Yeast:Mating pheromone response pathway/GPCR|GPCR]] | *[[Yeast:Mating pheromone response pathway/GPCR|GPCR]] | ||
*[[Yeast:Mating pheromone response pathway/Ras|Ras]] | *[[Yeast:Mating pheromone response pathway/Ras|Ras]] | ||
*[[Yeast:Mating pheromone response pathway/Pheromone|Pheromone]] | |||
==Miscellaneous== | ==Miscellaneous== | ||
*Crosstalk in the pathways is eliminated by formation of pathway-specific complexes (Ste5:Ste11:Ste7:Fus3, Pbs2:Ste11:Hog1) | *Crosstalk in the pathways is eliminated by formation of pathway-specific complexes (Ste5:Ste11:Ste7:Fus3, Pbs2:Ste11:Hog1) | ||
*In S. cerevisiae, meiosis is initiated only by diploid cells deprived of glucose and nitrogen and grown in the presence of a nonfermentable carbon source <cite>5</cite> | |||
===Pheromone processing genes=== | ===Pheromone processing genes=== | ||
*STE14 | *STE14 | ||
**carries out C-terminal methylation of prenylated proteins including a-factor, Ras1p, and Ras2p | **carries out C-terminal methylation of prenylated proteins including a-factor, Ras1p, and Ras2p | ||
**encodes the prenylcysteine-dependent carboxyl methyltransferase that mediates methylation, the final step in modification of CAAX proteins; Ste14p is also membrane associated | **encodes the prenylcysteine-dependent carboxyl methyltransferase that mediates methylation, the final step in modification of CAAX proteins; Ste14p is also membrane associated <cite>4</cite> | ||
*STE13 | *STE13 | ||
**Dipeptidyl aminopeptidase A involved in maturation of a-factor | |||
*AXL1 | *AXL1 | ||
**Protease involved in proteolytic step of a-factor N-terminal processing | |||
*STE23 | *STE23 | ||
*RAM1 | *RAM1, RAM2 | ||
* | **Required for a-factor prenylation | ||
*STE24 | *STE24 | ||
*RCE1 | *RCE1 | ||
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*STE23 | *STE23 | ||
===Other genes=== | |||
These genes are part of the mating machinery in yeast but not necessary part of the mating pheromone response pathway. | |||
*Swe1 - protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity | |||
*Ras1 - pombe homolog interacts with MAPKKK | |||
*SAG1 (α-agglutinin) - provides tight cell-cell adhesion during mating in S. cerevisiae. | |||
*FUS1 - protein with SH3 domain required for cell fusion during mating, located at the tip of the mating projection | |||
Ras1 - pombe homolog interacts with MAPKKK | |||
SAG1 (α-agglutinin) provides tight | |||
FUS1 | |||
==References== | ==References== | ||
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#2 pmid=9618441 | #2 pmid=9618441 | ||
#3 pmid=15374648 | #3 pmid=15374648 | ||
#4 pmid=8212897 | |||
#5 pmid=11248064 | |||
</biblio> | </biblio> |
Revision as of 15:46, 17 March 2006
Components
Miscellaneous
- Crosstalk in the pathways is eliminated by formation of pathway-specific complexes (Ste5:Ste11:Ste7:Fus3, Pbs2:Ste11:Hog1)
- In S. cerevisiae, meiosis is initiated only by diploid cells deprived of glucose and nitrogen and grown in the presence of a nonfermentable carbon source [1]
Pheromone processing genes
- STE14
- carries out C-terminal methylation of prenylated proteins including a-factor, Ras1p, and Ras2p
- encodes the prenylcysteine-dependent carboxyl methyltransferase that mediates methylation, the final step in modification of CAAX proteins; Ste14p is also membrane associated [2]
- STE13
- Dipeptidyl aminopeptidase A involved in maturation of a-factor
- AXL1
- Protease involved in proteolytic step of a-factor N-terminal processing
- STE23
- RAM1, RAM2
- Required for a-factor prenylation
- STE24
- RCE1
- KEX2
- KEX1
- STE23
Other genes
These genes are part of the mating machinery in yeast but not necessary part of the mating pheromone response pathway.
- Swe1 - protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity
- Ras1 - pombe homolog interacts with MAPKKK
- SAG1 (α-agglutinin) - provides tight cell-cell adhesion during mating in S. cerevisiae.
- FUS1 - protein with SH3 domain required for cell fusion during mating, located at the tip of the mating projection
References
- Pathway architecture overview
- Tzung KW, Williams RM, Scherer S, Federspiel N, Jones T, Hansen N, Bivolarevic V, Huizar L, Komp C, Surzycki R, Tamse R, Davis RW, and Agabian N. Genomic evidence for a complete sexual cycle in Candida albicans. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3249-53. DOI:10.1073/pnas.061628798 |
- Ashby MN, Errada PR, Boyartchuk VL, and Rine J. Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase. Yeast. 1993 Aug;9(8):907-13. DOI:10.1002/yea.320090810 |
- Kofahl B and Klipp E. Modelling the dynamics of the yeast pheromone pathway. Yeast. 2004 Jul 30;21(10):831-50. DOI:10.1002/yea.1122 |
- Banuett F. Signalling in the yeasts: an informational cascade with links to the filamentous fungi. Microbiol Mol Biol Rev. 1998 Jun;62(2):249-74. DOI:10.1128/MMBR.62.2.249-274.1998 |
- Bardwell L. A walk-through of the yeast mating pheromone response pathway. Peptides. 2004 Sep;25(9):1465-76. DOI:10.1016/j.peptides.2003.10.022 |