User:Isis Trenchard/Notebook/BioE44 Stuff/2010/03/24: Difference between revisions
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'''Day 2 (Thurs April 1)''' | '''Day 2 (Thurs April 1)''' | ||
*give them cultures with color generator vectors under inactive promotor? so no color expression | *give them cultures with color generator vectors <del>under inactive promotor? so no color expression</del> under constituative promotor so they can see colors and start thinking about how to measure input/output, understanding behavior etc. | ||
*miniprep | *miniprep | ||
*nanodrop | *nanodrop | ||
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*run gel | *run gel | ||
*cut bands, extract dna from gel | *cut bands, extract dna from gel | ||
*set up ligation (vectors already prepped by TAs - can choose type of inducible?) | *set up ligation (vectors already prepped by TAs - can choose type of inducible? either arabinose or iptg? high copy/low copy...) | ||
'''Day 3.5 (Wed April 7, TAs)''' | '''Day 3.5 (Wed April 7, TAs)''' |
Revision as of 14:57, 24 March 2010
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Reworking the first part of the class
Version 2 Day 2 (Thurs April 1)
Day 2.5 (Fri April 2, TAs)
Day 3 (Tues April 6)
Day 3.5 (Wed April 7, TAs)
Day 4 (Thurs April 8)
Day 4.5 (Fri April 9)
Day 4.5 (Mon April 12 - students)
Day 5 (Tues April 13)
Other stuff left to order
Pigment extraction protocol
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