User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/03/10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2011/03/10 Entry for User:J._M._Uriel_Urquiza/Notebook/pRK404_&_pK18Mobsacb_standardization) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Entry title== | ==Entry title== | ||
Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells. | |||
2µl of DNA | |||
100µl Cells | |||
1)30min on ice | |||
2)2 min 42ºC | |||
3)5 min on ice | |||
4)Add 900 µl LB | |||
5)1 hr. 37ºC | |||
6)Plate on LB Cb or Amp | |||
7)Incubate 37ºC overnight >16 gr. | |||
Revision as of 16:34, 10 March 2011
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleDistribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells. 2µl of DNA 100µl Cells 1)30min on ice 2)2 min 42ºC 3)5 min on ice 4)Add 900 µl LB 5)1 hr. 37ºC 6)Plate on LB Cb or Amp 7)Incubate 37ºC overnight >16 gr.
| |
