User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/03/10: Difference between revisions
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==Entry title== | ==Entry title== | ||
Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells. | Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells. | ||
Revision as of 16:34, 10 March 2011
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Entry titleDistribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells. 2µl of DNA 100µl Cells 1)30min on ice 2)2 min 42ºC 3)5 min on ice 4)Add 900 µl LB 5)1 hr. 37ºC 6)Plate on LB Cb or Amp 7)Incubate 37ºC overnight >16 gr.
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