User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/03/10

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==Entry title==
==Entry title==
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This document describes the standardization of pK18mobsacB & pRK404 for iGEM proposes.
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Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells.
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2µl of DNA
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100µl Cells
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1)30min on ice
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2)2 min 42ºC
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3)5 min on ice
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4)Add 900 µl LB
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5)1 hr. 37ºC
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6)Plate on LB Cb or Amp
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7)Incubate 37ºC overnight >16 gr.
   
   

Revision as of 18:31, 10 March 2011

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Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells. 2µl of DNA 100µl Cells 1)30min on ice 2)2 min 42ºC 3)5 min on ice 4)Add 900 µl LB 5)1 hr. 37ºC 6)Plate on LB Cb or Amp 7)Incubate 37ºC overnight >16 gr.



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