User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/03/10

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==Entry title==
==Entry title==
Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells.
Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells.
 +
2µl of DNA
2µl of DNA
 +
100µl Cells
100µl Cells
 +
1)30min on ice
1)30min on ice
 +
2)2 min 42ºC
2)2 min 42ºC
 +
3)5 min on ice
3)5 min on ice
 +
4)Add 900 µl LB
4)Add 900 µl LB
 +
5)1 hr. 37ºC
5)1 hr. 37ºC
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6)Plate on LB Cb or Amp
6)Plate on LB Cb or Amp
 +
7)Incubate 37ºC overnight >16 gr.
7)Incubate 37ºC overnight >16 gr.

Revision as of 19:32, 10 March 2011

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Entry title

Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells.

2µl of DNA

100µl Cells

1)30min on ice

2)2 min 42ºC

3)5 min on ice

4)Add 900 µl LB

5)1 hr. 37ºC

6)Plate on LB Cb or Amp

7)Incubate 37ºC overnight >16 gr.



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