User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/04/10: Difference between revisions
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The sequence of pSB1T3 was obtained form the Registry. This plasmid backbone can be found in 2010 Kit Plate 1 Well 7A. This plasmid instance carries a Red fluorescent protein generator denominated BBa_J04450, I also obtained its sequence from the Registry. | The sequence of pSB1T3 was obtained form the Registry. This plasmid backbone can be found in 2010 Kit Plate 1 Well 7A. This plasmid instance carries a Red fluorescent protein generator denominated BBa_J04450, I also obtained its sequence from the Registry. | ||
I have merged the sequences to design primers that would allows us to amplify a plasmid region. This | I have merged the sequences to design primers that would allows us to amplify a plasmid region. This amplified region would be used to standardize the vectors pK18mobsacB, pRK404 & pBBRMCS. | ||
Method. | Method. | ||
Revision as of 21:16, 10 April 2011
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Primer designThe sequence of pSB1T3 was obtained form the Registry. This plasmid backbone can be found in 2010 Kit Plate 1 Well 7A. This plasmid instance carries a Red fluorescent protein generator denominated BBa_J04450, I also obtained its sequence from the Registry. I have merged the sequences to design primers that would allows us to amplify a plasmid region. This amplified region would be used to standardize the vectors pK18mobsacB, pRK404 & pBBRMCS. Method. 1)
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