User:Jacob Esenther/Notebook/581/2014/09/24

Biomaterials Design Lab

Main project page Previous entry      Next entry

Objective Finished film synthesis from 09/19 Made two new films using ion-exchanged clay Run AA on arsenic samples Runs for arsenic and mercury samples are temporarily postponed due to technical issues such as defects with the detector and burnt out arsenic lamp. Lead (II) acetate · 3 H2O was cancelled as well. Description Finished film synthesis from 09/19 Synthesis procedure can be viewed here. Made two new films using ion-exchanged clay Film synthesis can be viewed here. We used our exchanged clay and Melvin's group's exchanged clay Our ion exchange was with tributylhexadecylphosphonium bromide (TP) Melvin's exchanged with (1-hexadecyl)triphenylphosphonium bromide (TBHD) Suspension of Films in Rhodamine 6G The arsenic, mercury, and lead were replaced with rhodamine 6G. Stock dilutions of 159 μM rhodamine 6G into 1, 10, and 20 μM were prepared by James, Michael, and Eleni. The following calculations for the dilution of rhodamine 6G are as follows, taken from Michael Bible's entry: Data Ion-Exchanged Films Film 1: 1.00888g PVA + 0.11630g TBHD Clay Film 2: 1.00123g PVA + 0.11024g other ion exchanged clay Preparation of solutions The three concentrations of R6G were created from a stock solution of 159 μM R6G. 1μM R6G - 0.629 mL of stock into 100mL - prepared by Eleni 10μM R6G - 6.289 mL of stock into 100mL - prepared by Michael 20μM R6G - 12.578mL of stock into 100mL - prepared by James Calculations V1*C1 = V2C2 V1 = V2C2/C1 Vstock = (100 mL)(1μM)/159μM) = 0.629 mL Vstock = (100 mL)(10μM)/159μM) = 6.289 mL Vstock = (100 mL)(20μM)/159μM) = 12.578 mL Vial Data ' R6G (uM) ' Empty Vial (g) Vial + Film (g) Film (g) PVA 1 13.04914 13.17062 0.12148 10 13.11216 13.27025 0.15809 20 13.08356 13.24444 0.16088 PVA-Clay 1 13.14655 13.2752 0.12865 10 13.11615 13.25209 0.13594 20 13.10316 13.2905 0.18734 Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.