User:Jacob Esenther/Notebook/581/2014/10/24: Difference between revisions
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==Objective== | ==Objective== | ||
# Make a column for column chromatography | |||
==Description== | ==Description== | ||
# | # Packing the Column | ||
## To pack the column we got a syringe with a stop cock at the bottom. | |||
## We packed the column with glass wool and washed it with methanol to help pack. | |||
## Next, a layer of sand was put on top | |||
## A solution of 9.9999g of Silica in methanol was poured into the column. | |||
##* The column must settle. | |||
## After draining the column was washed with DI water until the column ran clear. | |||
## Then 10mL of BPA and let it run into the layer of silica. | |||
## then 10mL of water, then 30mL of methanol were run into the column | |||
##* These were divided into 8 5mL fractions. | |||
==Data== | ==Data== |
Revision as of 11:28, 24 October 2014
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Objective
Description
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