User:Jacob Esenther/Notebook/Chem 571/2014/08/27

Biomaterials Design Lab

Main project page Next entry

Objective Preparation of Stock Solutions Stock solutions of a protein (lysozyme), and HAuCl4 were prepared. The stock solutions were used to make six solutions of varying concentrations containing HAuCl4, lysozyme, and H20. Description Solutions of Colloidal Gold and Lysosyme were produced at different ratios to observe the formation of fibers Solutions were heated in a hot water bath and run through the Uv-vis to observe any color changes. Solutions were also cooked within the oven for an extended period of time to assist fiber formation. Kinetics of AuNP Formation Enough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl4 to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution). The test tubes were covered with foil and placed in an 80°C hot water bath. Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer. The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained. Data Stock Solutions HAuCl4 → 0.04621g / 50ml DI Water Lysozyme → 0.0895g / 50ml DI Water Notes Using a teflon coated spatula is recommended so the HAuCl4 does not bind to the metal on the spatula. During the heating process, the temperature fluctuated between 70-85°C