User:Jacob Esenther/Notebook/Chem 571/2014/08/27: Difference between revisions
From OpenWetWare
(→Notes) |
(fix raw html notebook nav) |
||
(4 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 16: | Line 16: | ||
==Description== | ==Description== | ||
# | # Solutions of Colloidal Gold and Lysosyme were produced at different ratios to observe the formation of fibers | ||
## Solutions were heated in a hot water bath and run through the Uv-vis to observe any color changes. | |||
## Solutions were also cooked within the oven for an extended period of time to assist fiber formation. | |||
# Kinetics of AuNP Formation | |||
## Enough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl<sub>4</sub> to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution). | |||
## The test tubes were covered with foil and placed in an 80°C hot water bath. | |||
## Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer. | |||
## The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained. | |||
==Data== | ==Data== | ||
* | Stock Solutions | ||
* HAuCl4 → 0.04621g / 50ml DI Water | |||
* Lysozyme → 0.0895g / 50ml DI Water | |||
[[Image:Gold_-_DI_concentration_Table.jpg]] | |||
==Notes== | ==Notes== | ||
Using a teflon coated spatula is recommended so the HAuCl4 does not bind to the metal on the spatula. | Using a teflon coated spatula is recommended so the HAuCl4 does not bind to the metal on the spatula. | ||
During the heating process, the temperature fluctuated between 70-85°C | |||
[[Category:Course]] | [[Category:Course]] |
Latest revision as of 00:13, 27 September 2017
Biomaterials Design Lab | Main project page Next entry |
ObjectivePreparation of Stock Solutions Stock solutions of a protein (lysozyme), and HAuCl4 were prepared. The stock solutions were used to make six solutions of varying concentrations containing HAuCl4, lysozyme, and H20. Description
DataStock Solutions
NotesUsing a teflon coated spatula is recommended so the HAuCl4 does not bind to the metal on the spatula. During the heating process, the temperature fluctuated between 70-85°C
|