Take the UV-VIS spectra of amino acid solutions that were produced on September 3.
Fluorescence of Amino Acids
Repeat kinetics experiment originally preformed on August 27
Description
Take the UV-VIS spectra of amino acid solutions that were produced on September 3.
2-3 mL samples of each of the amino acid stock solutions were loaded into polystyrene cuvettes, one at a time, and the data gathered was used to create the graphs below.
Fluorescence of Amino Acids
A fluorescence spectrum of 81.2nM L-Tryptophan was run at a wavelength of 275nm.
the concentration used resulted from a 2500:1 dilution of a 203μM solution of L-Tryptophan
L-Tryptophan was the only amino acid that produced significant results with UV-VIS, hence the only one that required a fluorescence
Repeated kinetics experiment
0.5mL HAuCl4 was put in 5 test tubes
enough lysozyme was added to make a [Au]:[Lysozyme]=30 solution.
The chart in the notes segment illustrates concentrations necessary.
Test tubes were covered with foil and placed in an 80°C hot water bath.
Measurements were taken every 20 minutes on the UV-VIS
Measurements were taken from 800-260nm
Data
Amino Acid Absorbance Spectrum
Kinetics spectrum
Notes
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