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Objective
- Dialysis of protein and colloid against different dialyzing solutions
Description
- Dialysis
- prepared 500mL of 50mM NaCl
- prepared 1L of 50mM Glycine buffer, pH 3.5
- used 0.4mL 0.95M HCl and 0.37513 g glycine
- Prepared 25mL of 1g/L Lysozyme
- Dialyzed 4mL of lysozyme in about 3 inches of dialysis tubing
- prepared a 3500 g/mol MWCO tube for NaCl, Glycine, and HPLC water soaks
- prepared a 25k g/mol MWCO tube for Glycine Soak
- also dialyzed 4mL of a colloid solution in 3500 g/mol MWCO tube for Glycine soak
- Creating Calibration Curves
- Calibration curves of pH and conductivity were created for both NaCl and Glycine buffer
- 15mL of 2x, 5x, 10x, 50x, 100x, 200x, and 500x dilutions
Data
- Add data and results here...
Notes
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Biomaterials Design Lab
