User:Jacob Esenther/Notebook/Chem 571/2014/11/12: Difference between revisions
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==Data== | ==Data== | ||
# Bradford Analysis | # Bradford Analysis | ||
[[Image:Graphic_of_Bradford_Lysozyme.jpg|Graph plotting Absorbance of Lysozyme at differing Wavelengths]] | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''y=0.0206x+0.0969''' | |||
|- | |||
| ||Abs. at 590nM||Concentration of Lysozyme (ug/mL) | |||
|- | |||
| Bradford Blank||0.204||5.199029126 | |||
|- | |||
| 5uM CaCl2||0.46||17.62621359 | |||
|- | |||
| 50uM CaCl2||0.467||17.96601942 | |||
|- | |||
| 500uM CaCl2||0.431||16.2184466 | |||
|- | |||
| 5mM CaCl2||0.466||17.91747573 | |||
|- | |||
| 50mM CaCl2||0.43||16.16990291 | |||
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| | |||
|} | |||
* Table Displaying Calculated Concentrations based off of a Calibration curve of absorbance against a known concentration of Lysozyme. | |||
# UV Protein Analysis | # UV Protein Analysis | ||
[[Image:Graphic_of_UV_of_Lysozyme_571.jpg|Plot of absorbance of Lysozyme in the UV range of 200-400nm]] | |||
# Fluorescence Analysis | # Fluorescence Analysis | ||
[[Image:Fluorescence_of_Lysozyme_Graphic.jpg|Graph plotting Fluorescence at different Wavelength]] | |||
# ISC of CaCl<sub>2</sub> | # ISC of CaCl<sub>2</sub> | ||
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Always discuss methods between groups. It can only help you perfect your procedures. | Always discuss methods between groups. It can only help you perfect your procedures. | ||
[[Category:Course]] | [[Category:Course]] |
Revision as of 01:24, 14 November 2014
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Objective
Description
Data
NotesAlways discuss methods between groups. It can only help you perfect your procedures.
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