User:James C. Schwabacher/Notebook/CHEM-571/2013/09/04: Difference between revisions

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==Data: Group==
==Data: Group==
*Group Inosine Calibration
[[Image:CHEM571_cmj_09.04.13_Calibration_Inosine_Group.png]]
::'''Note''': None of the data points proved to be outliers for inosine.
*Group Adenosine Calibration With Outliers
*Group Adenosine Calibration With Outliers
[[Image:CHEM571_cmj_09.04.13_Calibration_Adenosine_Group_Outliers.png]]
[[Image:CHEM571_cmj_09.04.13_Calibration_Adenosine_Group_Outliers.png]]

Revision as of 12:44, 14 September 2013

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Objective

  1. Continue laboratory work from yesterday, collecting absorption data for adenosine and inosine solutions
  2. Calculate calibration curves using linear regression
  3. Conduct data analysis of the class' data: Means, Standard Deviations, Grubb's test for outliers, 95% and 90% Confidence Intervals

Procedure

  1. Procedure from yesterday was used for a second trial of adenosine. A new stock solution and set of dilutions were made. The stock solution was made from .0809 grams of solid adenosine:

Data: CMJ

Matt Hartings Why does some of this data go below zero? You should re-correct this. The baseline should be zero for this one.

Data: Group

  • Group Inosine Calibration

Note: None of the data points proved to be outliers for inosine.
  • Group Adenosine Calibration With Outliers

  • Group Adenosine Calibration Without Outliers

Note: Three data points were removed (2×105, 0.406; 2.5×105, 0.442; 3×105, 0.558). Removing the outliers decreased the slope from 14138 to 13411 and improved the r-squared value from .8921 to .9435.
  • Statistical Analysis of Group Data

Notes

All of the data collected yesterday was found to be systematically lower than the rest of the class' data. Thus, the data from the inosine trial was not included in the overall group data. Furthermore, a trial 2 of adenosine dilutions was run, using a new stock solution. This second set of data fit with the overall group data, and our first trial was discarded. It is most probable that there was an error in making the first set of stock solutions yesterday that resulted in the incomplete transfer of the massed solids into the 100 mL volumetric flasks. Having a smaller amount of solute in the solvent than calculated would result in systematically lower absorbance results.


Matt Hartings Where is the group data (in graph form)? Which data points did you get rid of? What did that do to the group's calibration curve? What is the concentration of the unknown you measured? What is the error on this measurement based on the error inherent in the calibration curve? Is your concentration in agreement with the concentration measured by the group that made it?


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