User:James C. Schwabacher/Notebook/CHEM-571/2014/02/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 69: Line 69:
==Data==
==Data==
* UV-Vis  
* UV-Vis  
[[Image:Bsa-cys.png]]




Revision as of 18:53, 25 February 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Characterize the previously synthesized BSA-L-cysteine ZnS:Mn Nanocrystals using UV-Vis.

Protocol

  1. Remove 1 mL aliquot from test tube and place in the cuvette.
  2. Take the UV-Vis spectra of the sample.
  3. Take the fluorescence spectra of each sample
  4. Repeat for each test tube
  5. Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate.
  6. Data analysis of the UV-Vis absorbances, and fluorescence spectra
  1. Determine if any changes in procedure need to be made.
    1. Is a different scale of protein:ligand ratio required?
    2. Were QD NCs successfully synthesized?
  2. Prepare stock solutions for next synthesis on the following day.

Note: Much of the protocol for today will be determined by the results of our first synthesis.

Edited Protocol

  • we are going to increase the final volume of solution to yield more mass to be collected. The original final volume will be multiplied by three.


Corrected Volumes Table

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 41.65 mM Ammonium Acetate (uL) 11uM BSA* (uL) 10uM L-Cysteine (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
0 540 60 600 0 600 3000 600 5.4
10 540 60 600 60 540 3000 600 5.4
20 540 60 600 120 480 3000 600 5.4
30 540 60 600 180 420 3000 600 5.4
40 540 60 600 240 360 3000 600 5.4
50 540 60 600 300 300 3000 600 5.4
60 540 60 600 360 240 3000 600 5.4
70 540 60 600 420 180 3000 600 5.4
80 540 60 600 480 120 3000 600 5.4
90 540 60 600 540 60 3000 600 5.4
100 540 60 600 600 0 3000 600 5.4

Data

  • UV-Vis


  • Fluorescence

Notes

  • Add before and after photos, and get uv-light photo.

The solutions appeared clear with the formation of white precipitate at the bottom of every test tube. 0% BSA had the most, while 100% BSA had the least. The precipitate emitted orange light when in the presence of ultra-violet rays.




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:James_C._Schwabacher/Notebook/CHEM-571/2014/02/04&oldid=776923"