User:James C. Schwabacher/Notebook/CHEM-571/2014/03/18: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
To begin collecting all data and based on our results refine the protocol for the rest of the semester.  


==Description==
==Table of Volumes for Large Synthesis==
# Add experimental record here. Include what, how, and why...
'''80% BSA-Cysteine'''
 
{| {{table}}
==Data==
| align="center" style="background:#f0f0f0;"|'''% BSA'''
* Add data and results here...
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)'''
==Notes==
| align="center" style="background:#f0f0f0;"|'''40 mM Ammonium Acetate (uL)'''
This area is for any observations or conclusions that you would like to note.
| align="center" style="background:#f0f0f0;"|'''10uM BSA* (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''10uM 1-Thioglycerol (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)'''
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)'''
 
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
[[Category:Course]]
|-
[[Category:Miscellaneous]]
| 80||10800||1200||12000||9600||2400||60000||12000||108
 
|}
 
 
[[Category:Course]]
[[Category:Miscellaneous]]


'''80% BSA-Cysteine, half concentration'''
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''% BSA'''
| align="center" style="background:#f0f0f0;"|'''2.5 mM Zinc Nitrate (uL)'''
| align="center" style="background:#f0f0f0;"|'''2.5 mM Manganese Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''20 mM Ammonium Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 uM BSA* (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 uM L-Cysteine (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 mM Ammonium Hydroxide (uL)'''
| align="center" style="background:#f0f0f0;"|'''2.5 mM Sodium Sulfide* (uL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 80||10800||1200||12000||9600||2400||60000||12000||108
|}


'''80% BSA-GSH'''
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''% BSA'''
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)'''
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''40 mM Ammonium Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM BSA* (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM GSH (uL)'''
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 80||10800||1200||12000||9600||2400||60000||12000||108
|}


'''80% BSA-1-Thioglycerol'''
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''% BSA'''
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)'''
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''40 mM Ammonium Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM BSA* (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM 1-Thioglycerol (uL)'''
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 80||10800||1200||12000||9600||2400||60000||12000||108
|}


==Preparation of Stock Solutions==
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''mass in grams (g)'''
| align="center" style="background:#f0f0f0;"|'''Final Volume (mL)'''
|-
| 5mM Zinc Nitrate||0.0745||50
|-
| 5mM Manganese Acetate||0.0123||10
|-
| 40mM Ammonium Acetate ||0.1541||50
|-
| 10uM BSA||0.033||50
|-
| 10mM Ammonium Hydroxide||168.92 (uL)||250
|-
| 5mM Sodium Sulfide||0.06||50
|}
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|}
|}


__NOTOC__
__NOTOC__

Latest revision as of 23:54, 26 September 2017

Biomaterials Design Lab Main project page
Previous entry      Next entry

Objective

To begin collecting all data and based on our results refine the protocol for the rest of the semester.

Table of Volumes for Large Synthesis

80% BSA-Cysteine

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM 1-Thioglycerol (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

80% BSA-Cysteine, half concentration

% BSA 2.5 mM Zinc Nitrate (uL) 2.5 mM Manganese Acetate (uL) 20 mM Ammonium Acetate (uL) 5 uM BSA* (uL) 5 uM L-Cysteine (uL) 5 mM Ammonium Hydroxide (uL) 2.5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

80% BSA-GSH

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM GSH (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

80% BSA-1-Thioglycerol

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM 1-Thioglycerol (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

Preparation of Stock Solutions

' mass in grams (g) Final Volume (mL)
5mM Zinc Nitrate 0.0745 50
5mM Manganese Acetate 0.0123 10
40mM Ammonium Acetate 0.1541 50
10uM BSA 0.033 50
10mM Ammonium Hydroxide 168.92 (uL) 250
5mM Sodium Sulfide 0.06 50


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