User:James C. Schwabacher/Notebook/CHEM-571/2014/11/05: Difference between revisions

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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
* PVA-NaMT Characterization
## Malachite Green absorption
## pXRD
## DSC


==Description==
*Mary's Bead Synthesis
# Add experimental record here. Include what, how, and why...
 
==Data==
* Add data and results here...
 
==Notes==
This area is for any observations or conclusions that you would like to note.


==Synthesis of PVA-clay Beads==
* 0.51520 g PVA + 0.05154 g NaMT in 6 mL 10% HCl.
* 750 uL of 0.8% glutaraldehyde was added to 20 mL of ethyl acetate. The mixture was stirred at maximum speed on a stirrer.
* The PVA-clay-HCl solution was added to the ethyl acetate mixture in a drop wise manner.
* 750 uL of 0.8% glutaraldehyde was added to 20 mL of ethyl acetate. The mixture was stirred at maximum speed on a stirrer.
* Depending on the conditions, microbeads should form between 2-3 minutes. As soon as the beads are visible, IMMEDIATELY ADD 25 mL of 0.2 M NaHCO<sub>3</sub>. Stir for at least 4 minutes, 15 minutes at most.
* Beads will be ready once a clear separation of the water and organic phase is observed. Beads will precipitate to the bottom of the beaker. There will still be some beads left in the organic solvent phase.
* Pour the contents into the falcon tube.
* Centrifuge for 1 min at 3000 rpm.
* Afterwards, remove the organic phase and suspend the beads in distilled water.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

Revision as of 07:04, 5 November 2014

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Objective

  • PVA-NaMT Characterization
    1. Malachite Green absorption
    2. pXRD
    3. DSC
  • Mary's Bead Synthesis

Synthesis of PVA-clay Beads

  • 0.51520 g PVA + 0.05154 g NaMT in 6 mL 10% HCl.
  • 750 uL of 0.8% glutaraldehyde was added to 20 mL of ethyl acetate. The mixture was stirred at maximum speed on a stirrer.
  • The PVA-clay-HCl solution was added to the ethyl acetate mixture in a drop wise manner.
  • 750 uL of 0.8% glutaraldehyde was added to 20 mL of ethyl acetate. The mixture was stirred at maximum speed on a stirrer.
  • Depending on the conditions, microbeads should form between 2-3 minutes. As soon as the beads are visible, IMMEDIATELY ADD 25 mL of 0.2 M NaHCO3. Stir for at least 4 minutes, 15 minutes at most.
  • Beads will be ready once a clear separation of the water and organic phase is observed. Beads will precipitate to the bottom of the beaker. There will still be some beads left in the organic solvent phase.
  • Pour the contents into the falcon tube.
  • Centrifuge for 1 min at 3000 rpm.
  • Afterwards, remove the organic phase and suspend the beads in distilled water.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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