User:James C. Schwabacher/Notebook/CHEM-571/2015/01/30: Difference between revisions

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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
*Malachite Green calibration curve
 
*redo PVA-Alg/PVA-Alg-NaMT bead synthesis, same scale, 4% CaCl, smaller beads using glass pipette
==Description==
# Add experimental record here. Include what, how, and why...
 
==Data==
* Add data and results here...
 
==Notes==
This area is for any observations or conclusions that you would like to note.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]


===Procedure===
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.>
Modified from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-582_Experimental_Chemistry_II/2015/01/28 1/28/2015]
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
#* 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
##* Add alginate '''while stirring''' PVA-water solution
#* ~0.1 g of nanocomposites should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of saturated citric acid with 2% calcium chloride
## Add 8.4 grams (4%) of calcium chloride to 210 mL of water
## Add citric acid to saturation (32 g citric acid)
##* 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
# Stir for 2 hrs to allow solidification
# Rinse with water to remove excess reactants


====Measurements====
*PVA-Alg
**PVA: 1.044 g
**Alginate: 0.16 g
**CaCl: 8.213 g
**citric acid: 32.01 g


[[Category:Course]]
*PVA-Alg-NaMT
[[Category:Miscellaneous]]
**PVA: 0.994 g
**Alg: 0.156 g
**NaMT: 0.103 g
**CaCl: 8.381 g
**citric acid: 33.01 g


*PVA-Alg-NaMT #2
**PVA: 1.098 g
**Alginate: 0.159 g
**NaMT: 0.125 g
**CaCl: 8.9 g
**citric acid: 32.02 g
**CaCl: 8.403 g
**citric acid: 32.04 g


*Vial, no cap: 13.023 g


====Notes====
*PVA-Alg does not work
*PVA-Alg-NaMT #2 made in sonicator


====Next Week====
*Madeleine: MG Calibration curve
*Eleni: Scale up PVA-Alg-NaMT synthesis
*James: start next nanocomposite synthesis
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__NOTOC__
__NOTOC__

Revision as of 13:16, 30 January 2015

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Objective

  • Malachite Green calibration curve
  • redo PVA-Alg/PVA-Alg-NaMT bead synthesis, same scale, 4% CaCl, smaller beads using glass pipette

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.> Modified from 1/28/2015

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of nanocomposites should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of saturated citric acid with 2% calcium chloride
    1. Add 8.4 grams (4%) of calcium chloride to 210 mL of water
    2. Add citric acid to saturation (32 g citric acid)
      • 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
  5. Stir for 2 hrs to allow solidification
  6. Rinse with water to remove excess reactants

Measurements

  • PVA-Alg
    • PVA: 1.044 g
    • Alginate: 0.16 g
    • CaCl: 8.213 g
    • citric acid: 32.01 g
  • PVA-Alg-NaMT
    • PVA: 0.994 g
    • Alg: 0.156 g
    • NaMT: 0.103 g
    • CaCl: 8.381 g
    • citric acid: 33.01 g
  • PVA-Alg-NaMT #2
    • PVA: 1.098 g
    • Alginate: 0.159 g
    • NaMT: 0.125 g
    • CaCl: 8.9 g
    • citric acid: 32.02 g
    • CaCl: 8.403 g
    • citric acid: 32.04 g
  • Vial, no cap: 13.023 g

Notes

  • PVA-Alg does not work
  • PVA-Alg-NaMT #2 made in sonicator

Next Week

  • Madeleine: MG Calibration curve
  • Eleni: Scale up PVA-Alg-NaMT synthesis
  • James: start next nanocomposite synthesis


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