User:James C. Schwabacher/Notebook/CHEM-571/2015/03/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2015/03/03 Entry for User:James_C._Schwabacher/Notebook/CHEM-571)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->


==March 3, 2015==
*Repeat synthesis, less stir time


===Procedure===
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.>
<br.>
PVA-Alg-NaMT
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate)
#** Add alginate '''while stirring''' PVA-water solution
#* '''~0.1 g of NaMT''' should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of citric acid, calcium chloride solution
## Add '''12-15 g (5-7%) citric acid'''
## Add '''8.4 g (4%) calcium chloride'''
## Heat solution to 40°C while stirring
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
#Allow solution to cool to room temp while stirring for another 20 min
# Stir in '''24 g''' (10%) citric acid solution for 20 min
# Stir in '''14.9 g''' (0.5 M) sodium sulfate solution for 20 min
# Rinse with water to remove excess reactants
# Allow beads to dry in teflon plate overnight




==Objective==
====Measurements====
Learn how to maintain an OpenWetWare Notebook.
*PVA: 1.00 g
 
*Alg: 0.16 g
==Description==
*NaMT: 0.11 g
# Add experimental record here. Include what, how, and why...
*citric acid: 11.99 g
 
*CaCl2: 8.42 g
==Data==
*10% citric acid: 24.0 g
* Add data and results here...
*sodium sulfate: 14.87 g
 
==Notes==
This area is for any observations or conclusions that you would like to note.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 


====Notes====
*Consider shortening time that beads stir in sodium sulfate
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Latest revision as of 00:48, 27 September 2017

Biomaterials Design Lab Main project page
Previous entry      Next entry

March 3, 2015

  • Repeat synthesis, less stir time

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of citric acid, calcium chloride solution
    1. Add 12-15 g (5-7%) citric acid
    2. Add 8.4 g (4%) calcium chloride
    3. Heat solution to 40°C while stirring
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
  5. Allow solution to cool to room temp while stirring for another 20 min
  6. Stir in 24 g (10%) citric acid solution for 20 min
  7. Stir in 14.9 g (0.5 M) sodium sulfate solution for 20 min
  8. Rinse with water to remove excess reactants
  9. Allow beads to dry in teflon plate overnight


Measurements

  • PVA: 1.00 g
  • Alg: 0.16 g
  • NaMT: 0.11 g
  • citric acid: 11.99 g
  • CaCl2: 8.42 g
  • 10% citric acid: 24.0 g
  • sodium sulfate: 14.87 g

Notes

  • Consider shortening time that beads stir in sodium sulfate


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:James_C._Schwabacher/Notebook/CHEM-571/2015/03/03&oldid=1016461"