March 3, 2015
- Repeat synthesis, less stir time
Procedure
Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.>
<br.>
PVA-Alg-NaMT
- Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
- 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
- Add alginate while stirring PVA-water solution
- ~0.1 g of NaMT should be added to this mixture where applicable
- Cool dissolved and mixed solution to 35°C
- Prepare 210 mL of citric acid, calcium chloride solution
- Add 12-15 g (5-7%) citric acid
- Add 8.4 g (4%) calcium chloride
- Heat solution to 40°C while stirring
- Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
- Allow solution to cool to room temp while stirring for another 20 min
- Stir in 24 g (10%) citric acid solution for 20 min
- Stir in 14.9 g (0.5 M) sodium sulfate solution for 20 min
- Rinse with water to remove excess reactants
- Allow beads to dry in teflon plate overnight
Measurements
- PVA: 1.00 g
- Alg: 0.16 g
- NaMT: 0.11 g
- citric acid: 11.99 g
- CaCl2: 8.42 g
- 10% citric acid: 24.0 g
- sodium sulfate: 14.87 g
Notes
- Consider shortening time that beads stir in sodium sulfate
|
Biomaterials Design Lab
