User:James C. Schwabacher/Notebook/CHEM-571/2015/03/06

Biomaterials Design Lab

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March 6, 2015 Repeat synthesis using a syringe and syringe pump Procedure Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) Add alginate while stirring PVA-water solution ~0.1 g of NaMT should be added to this mixture where applicable Cool dissolved and mixed solution to 35°C Prepare 210 mL of citric acid, calcium chloride solution Add 12-15 g (5-7%) citric acid Add 8.4 g (4%) calcium chloride Heat solution to 40°C while stirring Add the PVA-Alg-Nanocomposite mixture to a 10 ml syringe, carefully removing all air before tightening on the syringe tip Place the loaded syringe on the syringe pump, positioning the needle tip over the edge of the prepared citric acid/calcium chloride solution Set the syringe pump and press "run." The PVA solution will be added drop-wise to the cross-linking reaction mixture. Allow solution to cool to room temp while stirring for another 20 min Stir in 24 g (10%) citric acid solution for 30 min Stir in 14.9 g (0.5 M) sodium sulfate solution for 10 min Rinse with water to remove excess reactants Allow beads to dry in teflon plate overnight Measurements PVA: 1.01 g Alg: 0.16 g NaMT: 0.18 g citric acid: 12.98 g CaCl2: 8.40 g citric acid: 24.50 g sodium sulfate: 14.95 g Syringe Pump Settings B-D 10 mL Plastic Syringe 20 gauge syringe tip Volume: 7.8 Rate: 1 mL/min Notes