User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/30: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==Entry title==
==Objective==
* Insert content here...
* UVVis and Fluorescence of re-suspended JCS 1 and JCS 2
* Creation of a hot-injection version of this HgS QD synthesis
 
==Protocol==
#New BSA Solution (15 mg/mL)
## .1515 g of BSA in 10.1 mL of HPLC grade water
# Added 5 mL of 5mM HgNO<sub>3</sub> to 5 mL of 15 mg/mL BSA
# Added 60 μL of 1 M NaOH
# JCS 4 is being synthesized at room temperature with vigorous stirring
# JCS 5 is being synthesized at ~50 degrees Celsius (mantle setting 14.5) with vigorous stirring
 
==JCS 1 and JCS 2 Data==
[[Image:BSA_HgS_QDs_FL_100114.png]]
[[Image:BSA_HgS_QDs_UVVIS_10012014.png]]
[[Image:BSA_HgS_QDs_UVVIS_zoomed_10012014.png]]
 
==Data updated with JCS 3==
[[Image:BSA_HgS_QDs_FL_100114_2.png]]
[[Image:BSA_HgS_QDs_UVVIS_10012014_2.png]]
[[Image:BSA_HgS_QDs_UVVIS_zoomed_10012014_2.png]]
 
==Notes==
 
*The supernatant from the centrifugation of JCS 1 darkened to brown after sitting in the dark in the drawer
*After speaking with Dr. Hartings, we are going to try to rotovap the water off of the NPs, rather than the precipitation-centrifugation purification procedure.




Latest revision as of 00:21, 27 September 2017

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Objective

  • UVVis and Fluorescence of re-suspended JCS 1 and JCS 2
  • Creation of a hot-injection version of this HgS QD synthesis

Protocol

  1. New BSA Solution (15 mg/mL)
    1. .1515 g of BSA in 10.1 mL of HPLC grade water
  2. Added 5 mL of 5mM HgNO3 to 5 mL of 15 mg/mL BSA
  3. Added 60 μL of 1 M NaOH
  4. JCS 4 is being synthesized at room temperature with vigorous stirring
  5. JCS 5 is being synthesized at ~50 degrees Celsius (mantle setting 14.5) with vigorous stirring

JCS 1 and JCS 2 Data

Data updated with JCS 3

Notes

  • The supernatant from the centrifugation of JCS 1 darkened to brown after sitting in the dark in the drawer
  • After speaking with Dr. Hartings, we are going to try to rotovap the water off of the NPs, rather than the precipitation-centrifugation purification procedure.



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