User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2015/02/27: Difference between revisions
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* Adapted from [http://onlinelibrary.wiley.com/doi/10.1002/smll.201200760/full Goswami et. al] | * Adapted from [http://onlinelibrary.wiley.com/doi/10.1002/smll.201200760/full Goswami et. al] | ||
* We set out to synthesize mercury-sulfide quantum dots templated by Bovine serum albumin. Multiple attempts proved unsuccessful. However, in the process we discovered a reproducible synthesis that yields, what appears to be, a protein-based film suspended in aqueous solution. This page summarizes all of my experiments conducted during the 2014-2015 academic year, as well as my optimized synthesis procedure for producing these HgS-BSA films (aka the "Quick Synthesis"). | * We set out to synthesize mercury-sulfide quantum dots templated by Bovine serum albumin. Multiple attempts proved unsuccessful. However, in the process we discovered a reproducible synthesis that yields, what appears to be, a protein-based film suspended in aqueous solution. This page summarizes all of my experiments conducted during the 2014-2015 academic year, as well as my optimized synthesis procedure for producing these HgS-BSA films (aka the "Quick Synthesis"). | ||
* A few times throughout my notes I refer to the films formed as hydrogels or sol-gels. The sol-gels classification is incorrect, and, from what I can tell working with these materials, hydrogel may be the most appropriate category. | |||
* You will find UV-Vis and Fluorescence data for the first few syntheses (JCS 1-3) in an excel file on DropBox. This file also contains FTIR data from JCS 16. The sample was stuck to the membrane that was used to filter it, so a spectra of the membrane is also included. | * You will find UV-Vis and Fluorescence data for the first few syntheses (JCS 1-3) in an excel file on DropBox. This file also contains FTIR data from JCS 16. The sample was stuck to the membrane that was used to filter it, so a spectra of the membrane is also included. | ||
* There is a folder containing the DSC data for films JCS 4 and JCS 5 on DropBox. | * There is a folder containing the DSC data for films JCS 4 and JCS 5 on DropBox. |
Revision as of 12:24, 23 April 2015
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Project Summary
Original Protocol
"Quick" Protocol (15 min Synthesis)
Note: These solutions have a tendency to bump on the rotovap. Watch carefully and be ready to break vacuum temporarily in the beginning!Filtration ProtocolI conducted vacuum filtration once using a membrane filter (Supor-450, 47mm, 0.45μm). This worked to separate the gels from the solution, however do not leave the product on the membrane to dry--it will get stuck the membrane. Summary of Syntheses Attempted
Determining Sol-Gel formation factors
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