User:Jamie Nunziata/Notebook/Protease Research/2015/09/30: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objective==
==Objective==
The purpose of this lab is to use Bradford Assay to measure a-chymotrypsin degradation at a concentration of 1µM.
The purpose of this lab is to use Bradford Assay to measure AuNP fiber degradation from a solution of 1µM a-chymotrypsin protease
 


==Procedure==
==Procedure==


7 samples of AuNP fibers were spun down at 300rpm for 10 minutes. These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. The fiber samples had 975.6µL of buffer added to them. These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 24.5µL of 40.625µM a-chymotrypsin (in Tris) and 975.4µL of Tris buffer was added to each sample tube an a blank Eppindorf tube (no fibers), making the final solution 1µM a-chymotrypsin.
7 samples of AuNP fibers were spun down at 300rpm for 10 minutes.These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 24.5µL of 40.625µM a-chymotrypsin (in Tris) and 975.4µL of Tris buffer was added to each sample tube and a blank Eppindorf tube (no fibers), making the final solution 1µM a-chymotrypsin.




Line 24: Line 23:




Absorbance for each cuvette was measure via UV-Vis, and the data is recorded below
Absorbance for each cuvette was measure via UV-Vis between the emission sprectrum of 400-800nm, and the data is recorded below


==Data==
==Data==

Latest revision as of 01:20, 27 September 2017

Project name Main project page
Previous entry      Next entry

Objective

The purpose of this lab is to use Bradford Assay to measure AuNP fiber degradation from a solution of 1µM a-chymotrypsin protease

Procedure

7 samples of AuNP fibers were spun down at 300rpm for 10 minutes.These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 24.5µL of 40.625µM a-chymotrypsin (in Tris) and 975.4µL of Tris buffer was added to each sample tube and a blank Eppindorf tube (no fibers), making the final solution 1µM a-chymotrypsin.


To each cuvette, the following was added:

  • 1650µL of Tris buffer
  • 750µL of incubated 1µM sample (or blank)
  • 600µL of diluted Bradford Assay in Tris buffer (1:3)


A full lab procedure can be found in Nicole Bonan's notebook entry for today


Absorbance for each cuvette was measure via UV-Vis between the emission sprectrum of 400-800nm, and the data is recorded below

Data



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Jamie_Nunziata/Notebook/Protease_Research/2015/09/30&oldid=1017579"