User:Jamie Nunziata/Notebook/Protease Research/2015/10/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 21: Line 21:




A full lab procedure can be found in Nicole Bonan's notebook entry for [[User:Nicole_Bonan/Notebook/Chem_571_Lab_Notebook/2015/09/30|today]]
A full lab procedure can be found in Nicole Bonan's notebook entry for [[User:Nicole_Bonan/Notebook/Chem_571_Lab_Notebook/2015/10/20|today]]






Fluorescence for each sample was measured between the emission spectrum from 400 to 600nm, and the data is recorded below
Fluorescence for each sample was measured between the emission spectrum from 400 to 600nm, and the data is recorded below


==Data==
==Data==

Latest revision as of 01:21, 27 September 2017

Project name Main project page
Previous entry      Next entry

Objective

The objective of today's lab was to use a Fluorescence Assay to determine the rate a 100nM solution of a-chymotrypsin degrades AuNP fiber samples.

Procedure

7 samples of AuNP fibers were spun down at 300rpm for 10 minutes. These fibers will be used for our 120min, 90min, 60min, 45min, 30min, 15min, and 10min samples. 2.31µL of 43.359µM a-chymotrypsin (in phosphate buffer) and 997.7µL of phosphate buffer was added to each sample tube and a blank Eppindorf tube (no fibers), making a final solution of 100nM a-chymotrypsin.


To each cuvette, the following was added:

  • 20uL of the blank or sample
  • 140uL of Assay Buffer
  • 40uL of Assay Reagent


A full lab procedure can be found in Nicole Bonan's notebook entry for today


Fluorescence for each sample was measured between the emission spectrum from 400 to 600nm, and the data is recorded below

Data



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Jamie_Nunziata/Notebook/Protease_Research/2015/10/20&oldid=1017619"