User:Jarle Pahr/Characterising

Thoughts on characterisation of strains, plasmids and combinations thereof:

Types of characterisation that can be performed:

Growth rate.

Maximal cell density.

For fluorescent reporter constructs:

Note that depending on the stability of the fluorescent, fluorescence may correlate with recent gene activity, or total (accumulated) gene activity over the lifetime of the culture.

Fluorescence/gene expression displayed by saturated cultures.

Fluorescence displayed by exponentially growing cultures.

Fluorescence/gene expression displayed in different media.

Fluorescence/gene expression displayed for different incubation conditions (temperature, incubation in plate reader vs. shake flask, etc.)

Bibliography

http://sb6.biobricks.org/poster/automated-bioparts-characterisation-for-synthetic-biology/

The Spinach RNA aptamer as a characterisation tool for synthetic biology: http://pubs.acs.org/doi/abs/10.1021/sb400089c