User:Jarle Pahr/Checklists: Difference between revisions
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*For snap freezing: Dry ice or liquid nitrogen is available. | *For snap freezing: Dry ice or liquid nitrogen is available. | ||
*Freezer box is available | *Freezer box is available | ||
*'''Freezer box is marked'''. | |||
'''Electrophoresis:''' | '''Electrophoresis:''' | ||
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*After imaging: Save image as image file. Copy file(s) to personal area. | *After imaging: Save image as image file. Copy file(s) to personal area. | ||
*Remove gel from imaging apparatus. | *Remove gel from imaging apparatus. | ||
'''Gel extraction:''' | |||
*'''The correct band is cut.''' | |||
'''End of day:''' | '''End of day:''' | ||
*No samples are left on bench (that shouldn't be there). | *No samples are left on bench (that shouldn't be there). |
Revision as of 07:06, 12 May 2013
Checklists for lab procedures:
Transformation:
- Supercompetent cells are available (remove from freezer 20 min before use, to avoid waiting for cells to thaw).
- Enough plates, with right antibiotic, are available. Remove from cold storage, place in incubator for pre-heating.
- Waterbath for heat shock is at right temperature (42 C).
Before heat shock: Place plates in incubator for pre-heating.
Restriction digest:
- Needed restriction enzymes are available.
- Thaw buffer
- Waterbath at 37 C is available. (or otherwise, thermomixer, or otherwise, shaking incubator)
Ligation:
- Ligation buffer is available.
- Ligase is available.
- Correct ligase is used (T4 DNA ligase)
Preparation of agar plates:
- Magnetic stirrers are available
- Antibiotic is available. Thaw antibiotic.
Preparing supercompetent cells:
- Centrifuge is cooled
- Spectrophotometer and cuvettes for measuring OD are available
- When using spectrophotometer: Correct wavelength is selected (600 nm)
- For snap freezing: Dry ice or liquid nitrogen is available.
- Freezer box is available
- Freezer box is marked.
Electrophoresis:
- GelDoc is operational
- After imaging: Save image as image file. Copy file(s) to personal area.
- Remove gel from imaging apparatus.
Gel extraction:
- The correct band is cut.
End of day:
- No samples are left on bench (that shouldn't be there).