User:Jarle Pahr/Checklists

Checklists for lab procedures:

Transformation:

• Supercompetent cells are available (remove from freezer 20 min before use, to avoid waiting for cells to thaw).
• Enough plates, with right antibiotic, are available
• Waterbath for heat shock is at right temperature (42 C).

Before heat shock: Place plates in incubator for pre-heating.

Restriction digest:

• Needed restriction enzymes are available.
• Thaw buffer
• Waterbath at 37 C is available. (or otherwise, thermomixer, or otherwise, shaking incubator)

Ligation:

• Ligation buffer is available.
• Ligase is available.
• Correct ligase is used (T4 DNA ligase)

Preparing supercompetent cells:

• Centrifuge is cooled
• Spectrophotometer and cuvettes for measuring OD are available
• When using spectrophotometer: Correct wavelength is selected (600 nm)
• For snap freezing: Dry ice or liquid nitrogen is available.
• Freezer box is available