User:Jarle Pahr/DNA: Difference between revisions
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'''Analysis''' | '''Structure:''' | ||
http://www.chemguide.co.uk/organicprops/aminoacids/dna1.html | |||
'''Analysis and quantification''' | |||
http://nucleicacids.bitesizebio.com/articles/how-to-identify-supercoils-nicks-and-circles-in-plasmid-preps/ | http://nucleicacids.bitesizebio.com/articles/how-to-identify-supercoils-nicks-and-circles-in-plasmid-preps/ | ||
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http://www.pathologyoutlines.com/topic/moleculardnapuranalyzingpurity.html | http://www.pathologyoutlines.com/topic/moleculardnapuranalyzingpurity.html | ||
http://nanodrop.com/Library/CPMB-1st.pdf | |||
http://eu.idtdna.com/pages/decoded/decoded-articles/pipet-tips/decoded/2013/01/18/oligo-quantification-getting-it-right?utm_source=Twitter&utm_medium=Tweet&utm_campaign=decoded%2B3.1 | |||
'''Preticipation:''' | |||
http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=544&criteria= | |||
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http://nucleicacids.bitesizebio.com/articles/tech-clinic-3-dna-digestion-precipitation-and-clean-up/ | http://nucleicacids.bitesizebio.com/articles/tech-clinic-3-dna-digestion-precipitation-and-clean-up/ | ||
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/manip/conc.html | |||
http://bioinfoweb.com/Protocol-plasmid-DNA-isolation-by-alkaline-lysis-method-miniprep.htm | |||
http://www.mobio.com/blog/2013/03/27/where-oh-where-did-my-dna-go-a-post-on-dna-cleanup/ | |||
Comparison of alkaline-lysis protocols: | |||
'''Gel purification:''' | |||
Centrifugal filtration: http://www.zenbiology.com/2012/03/06/dna-extraction-from-agarose-gels-centrifugal-filtration/ | |||
http://www.ncbi.nlm.nih.gov/pubmed/10094803 | |||
Physical data: | Physical data: | ||
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http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/rna-tools-and-calculators/dna-and-rna-molecular-weights-and-conversions.html | http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/rna-tools-and-calculators/dna-and-rna-molecular-weights-and-conversions.html | ||
'''Amplification:''' | |||
PCR: | |||
LAMP: | |||
http://nar.oxfordjournals.org/content/28/12/e63.full | |||
MLPA: http://en.wikipedia.org/wiki/Multiplex_ligation-dependent_probe_amplification | |||
http://www.nito.no/Fagmiljoer/Bioingeniorfaglig-institutt/Bioingenioren/Alle-Bioingeniorene/Bioingenioren-2006/Bioingenioren-12-2006/Utproving-av-molekylaranalyse-av-mikrodelesjoner-ved-hjelp-av-MLPA/ | |||
'''Misc.:''' | |||
http://www.ehow.com/how_8554213_evaporate-dna-solution.html | |||
'''DNA elution from paper:''' | |||
http://wiki.answers.com/Q/How_do_you_elute_DNA_from_filter_paper | |||
http://2011.igem.org/Team:Cambridge/Protocols/Filter_Paper#/Protocols/Filter_Paper | |||
http://www.bio.net/bionet/mm/methods/2003-June/096480.html | |||
http://www.scribd.com/doc/23031907/Instructions-for-Elution-of-Plasmids-From-Whatman-Paper-2008 | |||
http://www.scientistsolutions.com/t10099-extraction+of+plasmid+dna+from+the+paper+.html | |||
http://www.protocol-online.org/biology-forums/posts/10829.html | |||
'''Storage and stability:''' | |||
'''DNA origami:''' | |||
http://cadnano.org/ | |||
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731887/ |
Latest revision as of 03:05, 16 July 2013
Structure:
http://www.chemguide.co.uk/organicprops/aminoacids/dna1.html
Analysis and quantification
http://nucleicacids.bitesizebio.com/articles/how-to-identify-supercoils-nicks-and-circles-in-plasmid-preps/
Quick reference - Determining DNA concentration and purity: http://nucleicacids.bitesizebio.com/articles/dna-concentration-purity/
http://www.pathologyoutlines.com/topic/moleculardnapuranalyzingpurity.html
http://nanodrop.com/Library/CPMB-1st.pdf
Preticipation:
http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=544&criteria=
Isolation:
DNA isolation methods anno 1999: http://rothlab.ucdavis.edu/protocols/DNA-from-Agarose-Review.html
Gel extraction by glass wool filtering: http://www.jcancer.org/v03p0093.htm
http://nucleicacids.bitesizebio.com/articles/10-tips-for-better-dna-gel-extraction-results/
http://nucleicacids.bitesizebio.com/articles/5-more-tips-for-dna-gel-extraction/
How alkalyine lysis works: http://bitesizebio.com/articles/the-basics-how-alkaline-lysis-works/
How to get better midiprep yields: http://nucleicacids.bitesizebio.com/articles/how-to-get-better-plasmid-midiprep-yields/
A menagerie of miniprep methods: http://bitesizebio.com/articles/a-menagerie-of-mini-prep-methods/
http://www.ncbi.nlm.nih.gov/pubmed/388356
A One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023457
http://nucleicacids.bitesizebio.com/articles/tech-clinic-3-dna-digestion-precipitation-and-clean-up/
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/manip/conc.html
http://bioinfoweb.com/Protocol-plasmid-DNA-isolation-by-alkaline-lysis-method-miniprep.htm
http://www.mobio.com/blog/2013/03/27/where-oh-where-did-my-dna-go-a-post-on-dna-cleanup/
Comparison of alkaline-lysis protocols:
Gel purification:
Centrifugal filtration: http://www.zenbiology.com/2012/03/06/dna-extraction-from-agarose-gels-centrifugal-filtration/
http://www.ncbi.nlm.nih.gov/pubmed/10094803
Physical data:
http://www.nordicbiosite.com/viewpage.aspx?pageid=33
Amplification:
PCR:
LAMP:
http://nar.oxfordjournals.org/content/28/12/e63.full
MLPA: http://en.wikipedia.org/wiki/Multiplex_ligation-dependent_probe_amplification
Misc.:
http://www.ehow.com/how_8554213_evaporate-dna-solution.html
DNA elution from paper:
http://wiki.answers.com/Q/How_do_you_elute_DNA_from_filter_paper
http://2011.igem.org/Team:Cambridge/Protocols/Filter_Paper#/Protocols/Filter_Paper
http://www.bio.net/bionet/mm/methods/2003-June/096480.html
http://www.scribd.com/doc/23031907/Instructions-for-Elution-of-Plasmids-From-Whatman-Paper-2008
http://www.scientistsolutions.com/t10099-extraction+of+plasmid+dna+from+the+paper+.html
http://www.protocol-online.org/biology-forums/posts/10829.html
Storage and stability:
DNA origami: