User:Jarle Pahr/Enzymes

Notes on various enzymes:

Polymerases (Not used in PCR. For polymerases used in PCR, see PCR page):

T4 DNA polymerase: Posesses 5' -> 3 DNA polymerase and 3' -> 5' exonuclease activity. Used in SLIC.

T5 Exonuclease: A 5' -'3 exonuclease. Used in Gibson Assembly.

Klenow fragment: Fragment of E. coli DNA polymerase I, retaining 5'-3' polymerase activity and 3’ → 5’ exonuclease activity, but without 5' → 3' exonuclease activity. The 3'->5' exonuclease activity of the Klenow fragment is roughly 200 times less than that of T4 DNA polymerase (http://www.vivo.colostate.edu/hbooks/genetics/biotech/enzymes/t4dnap.html). Klenow fragment is commonly used to generate blunt ends by overhang fill-in.

See also the book Enzymes of molecular biology