User:Jarle Pahr/Growth media: Difference between revisions
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See also http://en.wikipedia.org/wiki/Growth_medium | See also http://en.wikipedia.org/wiki/Growth_medium | ||
DM (Davis Minimal broth): Carlton, B. C., and B. J. Brown. 1981. Gene mutation. Pp. 222-242 in P. Gerhardt (ed.), Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C | |||
See also http://myxo.css.msu.edu/ecoli/dm25liquid.html | |||
Revision as of 12:05, 30 June 2013
Compositions of various growth media:
See also http://en.wikipedia.org/wiki/Growth_medium
DM (Davis Minimal broth): Carlton, B. C., and B. J. Brown. 1981. Gene mutation. Pp. 222-242 in P. Gerhardt (ed.), Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C
See also http://myxo.css.msu.edu/ecoli/dm25liquid.html
http://openwetware.org/wiki/Neidhardt_EZ_Rich_Defined
R2 agar: http://en.wikipedia.org/wiki/R2a_agar
Luria Broth/Lysogeny Broth (LB):
http://en.wikipedia.org/wiki/Lysogeny_broth
In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.)
According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3
LA:
To make LA plates, add 20 g agar/L
Psi-B medium:
M9 minimal medium:
Burks medium: https://media.vwr.com/stibo/hi_res/8287091.pdf
Bibliograpy
J Bacteriol. 1974 September; 119(3): 736–747. Culture Medium for Enterobacteria: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC245675/
http://schaechter.asmblog.org/schaechter/2009/11/the-limitations-of-lb-medium.html
Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full