User:Jarle Pahr/Growth media: Difference between revisions
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See also http://en.wikipedia.org/wiki/Growth_medium | |||
DM (Davis Minimal broth): Carlton, B. C., and B. J. Brown. 1981. Gene mutation. Pp. 222-242 in P. Gerhardt (ed.), Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C | |||
See also http://myxo.css.msu.edu/ecoli/dm25liquid.html | |||
http://openwetware.org/wiki/Neidhardt_EZ_Rich_Defined | |||
R2 agar: http://en.wikipedia.org/wiki/R2a_agar | |||
Luria Broth/Lysogeny Broth (LB): | |||
http://en.wikipedia.org/wiki/Lysogeny_broth | |||
TA agar: http://myxo.css.msu.edu/ecoli/taagar.html | |||
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EMB Agar: http://www.microbelibrary.org/component/resource/laboratory-test/2869-eosin-methylene-blue-agar-plates-protocol | |||
Burks medium: https://media.vwr.com/stibo/hi_res/8287091.pdf | |||
'''Media for spiroplasma and/or mycoplasma:''' | |||
M1D: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=400 | |||
=Supplies= | |||
Agar: http://www.kptkomet.no/produkter/no_89/biologi/vnr/800500-2/ | |||
http://www.formedium.com/europe/index.htm | |||
Peptone LP0037: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=LP0037 | |||
Yeast extract LP0021: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=LP0021&c=UK&lang=EN | |||
=Bibliograpy= | |||
J Bacteriol. 1974 September; 119(3): 736–747. Culture Medium for Enterobacteria: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC245675/ | |||
http://schaechter.asmblog.org/schaechter/2009/11/the-limitations-of-lb-medium.html | |||
Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full | Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full | ||
Latest revision as of 06:09, 22 August 2013
Compositions of various growth media:
See also http://en.wikipedia.org/wiki/Growth_medium
DM (Davis Minimal broth): Carlton, B. C., and B. J. Brown. 1981. Gene mutation. Pp. 222-242 in P. Gerhardt (ed.), Manual of methods for general bacteriology. American Society for Microbiology, Washington, D.C
See also http://myxo.css.msu.edu/ecoli/dm25liquid.html
http://openwetware.org/wiki/Neidhardt_EZ_Rich_Defined
R2 agar: http://en.wikipedia.org/wiki/R2a_agar
Luria Broth/Lysogeny Broth (LB):
http://en.wikipedia.org/wiki/Lysogeny_broth
TA agar: http://myxo.css.msu.edu/ecoli/taagar.html
In LB medium, exponential phase of E. coli has been estimated to end between OD600 0.6 - 1.0 (Sezonov et al.)
According to Sezonov et al., steady state (exponential growth) may end already at OD600 0.3
LA:
To make LA plates, add 20 g agar/L
Psi-B medium:
M9 minimal medium:
Burks medium: https://media.vwr.com/stibo/hi_res/8287091.pdf
Media for spiroplasma and/or mycoplasma:
M1D: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=400
Supplies
Agar: http://www.kptkomet.no/produkter/no_89/biologi/vnr/800500-2/
http://www.formedium.com/europe/index.htm
Peptone LP0037: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=LP0037
Yeast extract LP0021: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=LP0021&c=UK&lang=EN
Bibliograpy
J Bacteriol. 1974 September; 119(3): 736–747. Culture Medium for Enterobacteria: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC245675/
http://schaechter.asmblog.org/schaechter/2009/11/the-limitations-of-lb-medium.html
Sezonov et al. Escherichia coli Physiology in Luria-Bertani Broth: http://jb.asm.org/content/189/23/8746.full
