The BioRad Gene Pulser II electroporation system requires the Gene Pulser II Core unit and an acessory unit (Pulse Controller II or Capacitance Extander) The BioRad Gene Pulser II core unit (110 V) has been spotted on sale for 400 USD on EBay: http://www.ebay.com/itm/Bio-Rad-BioRad-Gene-Pulser-II-2-Apparatus-340BR-/290868541376?pt=LH_DefaultDomain_0&hash=item43b91db7c0 Shipping (~200 USD) and import tax would bring total cost to ~ 4500 NOK.

http://www.ebay.com/itm/BioRad-GenePulser-Gene-Pulser-II-Apparatus-Electroporation-System-/181067560006?pt=LH_DefaultDomain_0&hash=item2a2877b446

Pulse Controller spotted for ~200 USD:

http://www.ebay.com/itm/BIORAD-PULSE-CONTROLLER-BIO-RAD-1652098-FOR-GENE-PULSER-/280907232003?pt=UK_BOI_Medical_Lab_Equipment_Lab_Equipment_ET&hash=item4167603303

Recent development in low-cost electroporation: http://www.eurekalert.org/pub_releases/2013-02/f-sf-asb020713.php Appears to be only usable for electroporation of adherent cells.

List price for BioRad MicroPulser electroporator is 20 000 NOK: http://www.bio-rad.com/prd/en/NO/adirect/biorad?ts=1&cmd=BRCatgProductDetail&vertical=LSR&catID=83527990-34fb-4b33-b955-ca53b57bf8b9

Processors

http://en.wikipedia.org/wiki/ARM_architecture

Used in majority of smartphones.

http://en.wikipedia.org/wiki/X86

Electronics

http://en.wikipedia.org/wiki/Bug_Labs

Udoo: http://www.kickstarter.com/projects/435742530/udoo-android-linux-arduino-in-a-tiny-single-board

http://www.pcduino.com/

http://blog.makezine.com/2013/04/15/arduino-uno-vs-beaglebone-vs-raspberry-pi

http://freak.no/forum/forumdisplay.php?s=df6e715254c0b9035b3108b2ec531dbc&f=152

Arduino

Single-board microcontroller based on Atmel's ATMEGA8 and ATMEGA168 AVR microcontrollers. Arduino is a fork from the Wiring development platform.

http://en.wikipedia.org/wiki/Arduino

10 ways to destroy an arduino: http://www.ruggedcircuits.com/html/ancp01.html

Official resources

http://arduino.cc/

Introduction: http://www.arduino.cc/en/Guide/Introduction

FAQ: http://arduino.cc/en/Main/FAQ

Foundations: http://arduino.cc/en/Tutorial/Foundations

Language reference: http://arduino.cc/en/Reference/HomePage

Power supply: http://playground.arduino.cc/Learning/WhatAdapter

9-12 V DC
Minimum 250 mA, recommended 500mA - 1A.
2.1 mm power plug, centre positive

Arduino products: http://arduino.cc/en/Main/Products

Distributors: http://arduino.cc/en/Main/Products

Tutorials: http://arduino.cc/en/Tutorial/HomePage

Getting started: http://arduino.cc/en/Guide/HomePage

Software: http://arduino.cc/en/main/software

Runs on Windows, Macintosh OSX, and Linux operating systems.

http://arduino.cc/en/Reference/HomePage

Unofficial resources

MAKE Magazine - Arduino section: http://blog.makezine.com/category/arduino/

MAKE Magazine Arduino blog: http://blog.makezine.com/arduino/

http://www.arduinonorway.org/forum/

Clones and derivate products:

http://sanguino.cc/

SimDuino, an iPad Arduino simulator: http://arduino.cc/forum/index.php/topic,107629.0.html

Boards

Official Arduino boards:

Arduino Uno:

Microcontroller board based on ATmega328.
Can be powered by USB or external power supply.
14 digital input/output pins, 6 analog inputs. 16 MHz ceramic resonator.

Arduino Mini: http://arduino.cc/en/Main/ArduinoBoardMini

Arduino Mega 2560:

54 digital input/output pins, 16 analog inputs.
Compatible with most shields designed for the Arduino Duemilanove or Diecimila.

http://arduino.cc/en/Main/ArduinoBoardMega2560

http://www.liquidware.com/shop/show/AMEGA2560/Arduino+Mega+2560

http://www.makershed.com/Arduino_Mega_p/mksp5.htm

Unofficial boards:

Ruggeduino: http://www.ruggedcircuits.com/html/ruggeduino.html

Expansion shields

http://arduino.cc/en/Main/ArduinoShields

http://shieldlist.org/

http://www.adafruit.com/blog/2010/11/01/arduino-shields-an-adafruit-electronics-gift-guide/

http://www.makershed.com/SearchResults.asp?Search=shield

http://www.trossenrobotics.com/c/arduino-shields.aspx

http://www.liquidware.com/category/Arduino

http://www.mindsensors.com/index.php?module=pagemaster&PAGE_user_op=view_page&PAGE_id=144

Screens:

LOL shield:

http://www.makershed.com/LoL_shield_for_Arduino_p/mkjr3.htm

Digit shield: http://www.makershed.com/Digit_Shield_Green_p/mkntd3.htm

Audio:

http://www.makershed.com/Voice_Shield_kit_for_Arduino_p/mkskl3.htm

http://store.open-electronics.org/Arduino/Shield/AUDIO_SHIELD

Networking & communication:

http://arduino.cc/en/Main/ArduinoEthernetShield

http://arduino.cc/en/Main/ArduinoWirelessShield

http://arduino.cc/en/Main/ArduinoBoardBluetooth

http://arduino.cc/en/Main/ArduinoGSMShield

http://www.open-electronics.org/arduino-gsm-shield/

Motors:

http://arduino.cc/en/Main/ArduinoMotorShieldR3

http://www.ruggedcircuits.com/html/rugged_motor_driver.html

http://www.open-electronics.org/arduino-motor-shield/

Input:

Buttonshield: http://www.liquidware.com/shop/show/BUT/ButtonShield

Sensors:

GPS shield: http://www.ladyada.net/make/gpsshield/

GSM/GPRS and GPS shield: http://store.open-electronics.org/?_route_=/Arduino/Shield/GSM_GPRS_GPS_SHIELD

Weather shield: http://www.zipfelmaus.com/blog/arduino-weather-shield-schematics-layout-code-everything-you-need/

Weather shield: http://www.ethermania.com/shop/index.php?main_page=product_info&cPath=91_104&products_id=612

pHduino: http://code.google.com/p/phduino/

Power:

http://www.liquidware.com/shop/show/BPM/Mega+Backpack

Lithium backpack: http://www.liquidware.com/shop/show/MBP/Lithium+Backpack

Misc:

http://www.makershed.com/DrinkShield_for_Arduino_p/mkgx01.htm

http://www.cooking-hacks.com/index.php/documentation/tutorials/raspberry-pi-to-arduino-shields-connection-bridge

http://www.ruggedcircuits.com/html/gadget_shield.html

http://www.cooking-hacks.com/index.php/shop/arduino/shields/ehealth-sensor-shield-biometric-medical-arduino-raspberry-pi.html

MicroSD shield: http://www.sensor-networks.org/index.php?page=0827727742

Concepts

Sketch: The term for an Arduino program. A unit of code that is uploaded and run on an Arduino board. Sketches are saved with the file extension .ino.
Shields: Printed circuit expansion boards called "shields" can be plugged into the Arduino pin-headers to provide specialized functions such as GPS, ethernet, LCD display etc.

Programming

http://arduino.cc/en/Guide/Environment

The Arduino programming environment is based on the Processing programming environment (http://www.processing.org/).

The Arduino language is based on C/C++. After processing, Arduino codes is handed of to a C/C++ compiler (avr-g++).

http://blog.makezine.com/2013/04/15/developing-for-arduino-on-android/

Because Arduino uses Atmel's microprocessors, Atmels development environments AVR Studio and Atmel Studio may also be used for software development. Functions/methods:

Applications

Building Research Equipment with Free, Open-Source Hardware: http://www.sciencemag.org/content/337/6100/1303.summary

http://www.diydrones.com/notes/ArduPilot

http://playground.arduino.cc/Projects/Ideas

http://www.instructables.com/id/Arduino-Projects/

ArduPilot Mega - Arduino-based autopilot: http://code.google.com/p/ardupilot-mega/wiki/APM2board

GPS tracking: http://www.jeremyblum.com/2012/07/16/tutorial-15-for-arduino-gps-tracking/

Books

Programming Arduino: Getting started with Sketches: http://www.amazon.com/Programming-Arduino-Getting-Started-Sketches/dp/0071784225/ref=sr_1_3?ie=UTF8&qid=1366760346&sr=8-3&keywords=Arduino

Arduino Cookbook: http://www.amazon.com/Arduino-Cookbook-Michael-Margolis/dp/1449313876/ref=sr_1_7?ie=UTF8&qid=1366760346&sr=8-7&keywords=Arduino

Practical Arduino: Cool projects for open source hardware: http://www.amazon.com/Practical-Arduino-Projects-Hardware-Technology/dp/1430224770/ref=tag_dpp_lp_edpp_ttl_in

http://www.amazon.com/30-Arduino-Projects-Evil-Genius/dp/007174133X/ref=pd_sim_pc_5

http://www.robonor.no/mage/default/arduino/123-robotics-experiments-for-the-evil-genius.html

Accessories/Kits

http://no.rs-online.com/web/p/processor-microcontroller-development-kits/7617355/?

Sparkfun Inventors kit: http://www.amazon.com/Sparkfun-Inventors-professional-Arduino-retail/dp/B005FMNJ3S/ref=sr_1_14?ie=UTF8&qid=1366760346&sr=8-14&keywords=Arduino

Costs $100, free shipping.

Newsite Uno 3 starter kit: http://www.amazon.com/Starter-Kit-Newsite-Uno-Breadboard/dp/B0051QHPJM/ref=sr_1_2?ie=UTF8&qid=1366760346&sr=8-2&keywords=Arduino

Getting Started with Arduino Kit v3.0: http://www.makershed.com/Getting_Started_with_Arduino_Kit_V3_0_p/msgsa.htm

Costs $65

http://arduino.cc/en/Main/ArduinoStarterKit

http://store.arduino.cc/eu/index.php?main_page=product_info&cPath=2_23&products_id=185

Costs ~ 80€. ( + shipping + import duty)

Adafruit Budget Pack for Arduino (Arduino Uno R3) - Uno w/328: http://www.adafruit.com/products/193

Costs $50

Adafruit Starter Pack for Arduino (Includes Arduino Uno R3) - 1.0: http://www.adafruit.com/products/68

Costs $65

http://www.robonor.no/mage/default/arduino/arduino-inventors-kit-koffert.html

Sparkfun Inventors Kit (SIK) solgt av Robonor, for NOK 889,-

Raspberry Pi

http://en.wikipedia.org/wiki/Raspberry_Pi

Boots from SD card (Not included in price). Minimum size 2 GB. Minimum 4GB recommended. Pre-loaded SD cards can be bought. (for example: http://www.amazon.co.uk/For-Raspberry-preloaded-operating-partitioned/dp/B008IU78EK) If not, an SD card reader is needed to prepare the card. High-speed category 4 is recommended. See http://elinux.org/RPi_Easy_SD_Card_Setup and http://elinux.org/RPi_SD_cards
Programs must be compilable for ARMv6 architecture. (Windows/x86 not supported)
Video output through HDMI or Composite. No VGA.
Power through micro USB port. Standard phone charger with micro USB plug can be used (minimum 700mA at 5 volts).
No Real Time Clock (RTC) on board. Time is updated over internet after each boot.
Not all peripherals (keyboards, mice, etc.) may work (may draw too much power). See http://elinux.org/RPi_VerifiedPeripherals
Two models: A and B. B model draws 3.5 W, the A model 2 W.
B model specifications: 700 Mhz ARM1, 256 MB SDRAM

Official resources:

http://www.raspberrypi.org/

Official downloads: http://www.raspberrypi.org/downloads

Official forum - basic setup and usage: http://www.raspberrypi.org/phpBB3/viewforum.php?f=26

Quick start guide: http://www.raspberrypi.org/wp-content/uploads/2012/12/quick-start-guide-v1.1.pdf

FAQ: http://www.raspberrypi.org/faqs

Unofficial resources:

Unofficial wiki: http://elinux.org/RPi_Hub

Buying guide: http://elinux.org/RPi_Buying_Guide

Introducing the raspberry pi - getting started: http://www.h2g2.com/approved_entry/A9143796

Introducing the raspberry pi - peripherals you'll need: http://www.h2g2.com/approved_entry/A13413584

Raspberry Pi Stack exchange: http://raspberrypi.stackexchange.com/

The Pi Hut - accessories: http://thepihut.com/

http://blog.makezine.com/category/electronics/raspberry-pi/

"The true cost of Raspberry Pi - essential accessories": http://www.penguintutor.com/blog/viewblog.php?blog=6354

https://www.modmypi.com/

Gotcha's and new users tips: http://www.keyboardmaven.com/2013/04/raspberry-pi-gotchas-and-newbiebuyer.html

http://elinux.org/RPi_Hardware_Basic_Setup#Prepared_Operating_System_SD_Card

Applications:

http://www.digi.no/914772/denne-kontrolleres-med-rasperry-pi

http://www.theregister.co.uk/2013/03/14/feature_ten_raspberry_pi_projects/

http://blog.makezine.com/2013/04/14/47-raspberry-pi-projects-to-inspire-your-next-build/

Books:

Gray Girling: Rapsberry Pi Owners workshop manual: http://www.amazon.co.uk/Raspberry-Pi-Manual-practical-revolutionary/dp/0857332953

Mike McGrath: Raspberry Pi in easy steps: http://www.amazon.com/Raspberry-Easy-Steps-Mike-McGrath/dp/1840785810

Review of Raspberry pi books: http://www.theregister.co.uk/2013/04/18/feature_getting_into_the_raspberry_pi_books/

Richardson & Wallace. Getting Started with Raspberry Pi. Published December 2012: http://shop.oreilly.com/product/0636920023371.do

Upton & Halfacree: Raspberry Pi User Guide: http://www.amazon.com/Raspberry-User-Guide-Gareth-Halfacree /dp/111846446X Published September 2012. Somewhat dated already?

News and reviews:

http://www.itavisen.no/nyheter/raspberry-pi-er-helt-fantastisk-4976

Tinkerforge

http://en.wikipedia.org/wiki/Tinkerforge

http://www.tinkerforge.com/en

3D Printing

Filaments

ABS plastic

PLA: http://reprap.org/wiki/PLA

Food safe printing:

http://www.3dprintingera.com/food-safe-3d-printing/

http://www.dezeen.com/2013/03/27/food-is-the-next-frontier-of-3d-printing-janne-kytannen/

http://reprage.com/post/36869678168/is-3d-printed-pla-food-safe

Links

http://blog.makezine.com/category/workshop/3d-printing-workshop/

http://reprage.com/

http://www.shapeways.com/

http://www.3dprintingera.com/

http://www.3dprintingera.com/what-are-3d-printers-bad-at/

http://vort.org/2012/11/20/why-doesnt-your-lab-have-3d-printer-yet/

http://forums.somethingawful.com/showthread.php?threadid=3365193

http://whatabout.no/

http://www.reprapcentral.com/vmchk.html

http://cubify.com/cube/index.aspx?hp_bn_cube

http://www.ultimaker.com/

www.3ders.org/

http://www.3ders.org/articles/20121123-meet-metalbot-new-all-metal-reprap-compatible-3d-printer.html

http://www.robo3dprinter.com/

http://www.thingiverse.com/

http://www.pp3dp.com/

http://www.lulzbot.com/catalog

http://vort.org/2011/09/19/how-sequence-10000-metagenomes-3d-printer/

Solidoodle

http://www.solidoodle.com/

Solidoodle 3D printer 3d generation: http://store.solidoodle.com/index.php?route=product/product&product_id=79

799 USD

Solidoodle 3D printer 2nd generation: http://store.solidoodle.com/index.php?route=product/product&product_id=56

499 USD

Makerbot

www.makerbot.com

Makerbot Replicator 2: http://store.makerbot.com/replicator2.html

2200 $ USD

RepRap

http://en.wikipedia.org/wiki/RepRap_Project

http://reprap.org/wiki/Main_Page

http://forums.reprap.org/list.php?1

http://www.reprap.org/wiki/Main_Page

http://reprap.org/wiki/RepRap_Buyers%27_Guide

http://www.robonor.no/mage/default/

http://www.3dstuffmaker.com/

http://www.makergear.com/products/3d-printers

http://reprappro.com/

Other printers

http://pinterest.com/finklean/diy-3d-printers/

http://makibox.com/products

http://makibox.com/details/product/A6-LT

200 USD. Uses PLA filament. 6-10 weeks lead time.

Software

http://replicat.org/

https://github.com/SolidCode/SolidPython#readme

Reviews and discussion

http://reviews.cnet.com/3d-printers/makerbot-replicator-3d-printer/4505-33809_7-35266602.html

http://freak.no/forum/showthread.php?t=219137

http://www.treehugger.com/gadgets/28-affordable-3d-printers-you-can-buy-now.html

http://blog.makezine.com/2013/03/25/will-3d-printers-save-the-world/

Vendors

http://www.reprapdiscount.com/

http://adafruit.com/

http://www.makershed.com/default.asp

http://www.raspberry-pi-buy.com/

https://www.sparkfun.com/

http://www.creativetools.se/

3D scanners

MakerScanner

http://www.makerscanner.com/

http://www.makerscanner.com/docs/1-makerscanner.html

http://www.thingiverse.com/thing:3946

http://abarry.org/makerscanner/2-makerscanner-building.html

DAVID 3D Scanner

http://www.david-laserscanner.com/

http://shop.david-vision-systems.de/

FabScan

FabScan: http://hci.rwth-aachen.de/fabscan

FabScan hardware: https://hci.rwth-aachen.de/fabscan_hardware

https://groups.google.com/forum/?fromgroups=#!forum/fabscan

https://github.com/francisengelmann/FabScan100

http://shop.mariolukas.de/de/Fabscan-Kit

FabScan 100 Linux Version: https://groups.google.com/forum/?fromgroups=#!topic/fabscan/oIMZYx_utjo

FabScan Arduino shield: http://hci.rwth-aachen.de/fabscan_shield

http://www.watterott.com/de/Arduino-FabScan-Shield

Ideas

Bacteria transformer:

Objective: Obtain automatation and decreased variability in the handling of cells during heat-shock transformation.

Input materials: Eppendorf tubes (containing supercompetent cells or DNA), water, ice, sterile liquid growth medium, pipette tips. Should perform: Add DNA, heat shock, addition of medium, incubation. Should produce: Transformed cells ready for plating out.

Technical requirements: Must be able to open and close eppendorf tubes. Must be able to regulate temperature for heat shock and incubation chambers/water baths. Must be able to transfer all tubes simultaneously. Must be able to transfer tubes rapidly between ice bath and heat shock bath.

Challenges: Opening/closing tubes. Tubes must be kept firmly in place when opening/closing lids. Either lid closing/opening operation must be very reliable, or the machine must be able to detect a failure to correctly open/close all lids (the latter option probably hard to implement).

Idea: Place all tubes in a single row on a movable rack (movable in direction y). For adding DNA and medium, use two standard autopipettes (volume must be adjusted manually) mounted to the side (direction x) of the rack. Use an overhead rail to move the pipettes over the tubes, using a stepper motor to ensure that the pipette stops at the correct position above each tube. Two additional motors are needed to operate the pipette: One to push the piston, and one to operate the pipette-tip discard mechanism. Use a lifting mechanism and rail system to move the rack between the water baths.

To open/close the tubes, use one or several metal or plastic bars mounted along the tube rack so that the lids rest on the bar (all tubes must be placed on the rack with the lid in the right orientation), the bar being connected to a rotation mechanism, which when rotated causes the lids to be opened. A similar mechanism can be used to close the tubes. For this to work, the tubes must be kept firmly in place and be well supported from below (otherwise, tubes will be pushed downwards without the lid closing properly).

Questions: How can the pipettes be moved? How can the rack be moved between the bath?

Needed components:

Motors Temperature sensors Heating elements Rails and movement mechanisms

Plate spreader:

Input materials: Plastic petri dishes with agar, sterile glass beads, eppendorf tubes containing cells.

Should perform:

Core functionality: Remove cover from plates, transfer cells to plates, add glass beads to plates, replace cover on plates, spread cells by shaking plastes.

Nice to have: Invert plates (while avoiding cover falling off), transfer plates to incubation chamber.

Milling and machining

http://blog.arduino.cc/2013/05/06/othermill-on-kickstarter/?utm_source=buffer&utm_medium=facebook&utm_campaign=Buffer&utm_content=buffer2e4db

http://www.kickstarter.com/projects/otherfab/the-othermill-custom-circuits-at-your-fingertips

User:Jarle Pahr/Hardware

Tablets

Lab equipment

Automated fluid handling

Microfluidics

Electroporation