User:Jarle Pahr/Plasmids: Difference between revisions
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! Plasmid!! Description !!Length (bp) !! Origin of Replication !! Selection markers!! Comment | ! Plasmid!! Description !!Length (bp) !! Origin of Replication !! Selection markers!! Data!! Comment | ||
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|pJP-1||Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI || ||oriV (RK2) ||KanR|| | |pJP-1||Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI || ||oriV (RK2) ||KanR|| || | ||
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|pJP-2||Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI ||6105 ||oriV (RK2) ||KanR|| | |pJP-2||Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI ||6105 ||oriV (RK2) ||KanR|| || | ||
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|pJP-3||Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6093 ||oriV (RK2) ||KanR|| | |pJP-3||Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6093 ||oriV (RK2) ||KanR|| || | ||
|- | |- | ||
|pJP-4||Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6091 ||oriV (RK2) ||KanR|| | |pJP-4||Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6091 ||oriV (RK2) ||KanR|| || | ||
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|pJP-5||Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| | |pJP-5||Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| || | ||
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|pJP-6||Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| | |pJP-6||Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| || | ||
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|pJP-7||Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| | |pJP-7||Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| || | ||
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|pJP-8||Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6118 ||oriV (RK2) ||KanR|| | |pJP-8||Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6118 ||oriV (RK2) ||KanR|| || | ||
|- | |- | ||
|pJP-9||Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6105 ||oriV (RK2) ||KanR|| | |pJP-9||Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6105 ||oriV (RK2) ||KanR|| || | ||
|- | |- | ||
|pJP-10||Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI ||6095 ||oriV (RK2) ||KanR|| | |pJP-10||Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI ||6095 ||oriV (RK2) ||KanR|| || | ||
|- | |- | ||
|pJP-11||Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI||7867||oriV (R2)||KanR|| | |pJP-11||Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI||7867||oriV (R2)||KanR|| || | ||
|} | |} | ||
Revision as of 09:56, 2 May 2013
| Plasmid | Description | Length (bp) | Origin of Replication | Selection markers | Data | Comment |
|---|---|---|---|---|---|---|
| pJP-1 | Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI | oriV (RK2) | KanR | |||
| pJP-2 | Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | ||
| pJP-3 | Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6093 | oriV (RK2) | KanR | ||
| pJP-4 | Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6091 | oriV (RK2) | KanR | ||
| pJP-5 | Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
| pJP-6 | Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
| pJP-7 | Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
| pJP-8 | Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6118 | oriV (RK2) | KanR | ||
| pJP-9 | Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | ||
| pJP-10 | Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI | 6095 | oriV (RK2) | KanR | ||
| pJP-11 | Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI | 7867 | oriV (R2) | KanR |
