User:Jarle Pahr/Plasmids: Difference between revisions
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=Links= | |||
http://histmicro.yale.edu/history1.htm | |||
=Bibliography= | |||
http://publisher.medfak.ni.ac.rs/2007-html/4-broj/BACTERIAL%20PLASMIDS.pdf | |||
http://mmbr.asm.org/content/62/2/434.full | |||
http://mic.sgmjournals.org/content/147/4/763.full.pdf | |||
Identification and Classification of Bacterial Plasmids: http://pubmedcentralcanada.ca/pmcc/articles/PMC373151/pdf/microrev00046-0077.pdf | |||
Microbiol Mol Biol Rev. 1998 June; 62(2): 434–464. PMCID: PMC98921. Replication and Control of Circular Bacterial Plasmids: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/ | |||
Identification of cellular objective for elucidating the physiological state of plasmid-bearing Escherichia coli using genome-scale in silico analysis.: http://www.ncbi.nlm.nih.gov/pubmed?cmd=Retrieve&list_uids=19224555 | |||
=Linear plasmids= | |||
http://link.springer.com/chapter/10.1007%2F7171_2007_095 | |||
=Supplies= | |||
pBR322: http://www.kptkomet.no/produkter/no_89/biologi/vnr/779616/ | |||
Latest revision as of 04:25, 22 August 2013
| Plasmid | Description | Length (bp) | Origin of Replication | Selection markers | Data | Comment |
|---|---|---|---|---|---|---|
| pJP-1 | Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI | oriV (RK2) | KanR | 6IE58_.fas | ||
| pJP-2 | Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | 76IE57_.fas | |
| pJP-3 | Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6093 | oriV (RK2) | KanR | ||
| pJP-4 | Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6091 | oriV (RK2) | KanR | ||
| pJP-5 | Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
| pJP-6 | Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
| pJP-7 | Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
| pJP-8 | Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6118 | oriV (RK2) | KanR | ||
| pJP-9 | Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | ||
| pJP-10 | Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI | 6095 | oriV (RK2) | KanR | ||
| pJP-11 | Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI | 7867 | oriV (R2) | KanR |
pSB-M1g
pUC19:
pUC118:
pUC128: http://www.ncbi.nlm.nih.gov/pubmed/2853689
pUM9
pUM66
Links
http://histmicro.yale.edu/history1.htm
Bibliography
http://publisher.medfak.ni.ac.rs/2007-html/4-broj/BACTERIAL%20PLASMIDS.pdf
http://mmbr.asm.org/content/62/2/434.full
http://mic.sgmjournals.org/content/147/4/763.full.pdf
Identification and Classification of Bacterial Plasmids: http://pubmedcentralcanada.ca/pmcc/articles/PMC373151/pdf/microrev00046-0077.pdf
Microbiol Mol Biol Rev. 1998 June; 62(2): 434–464. PMCID: PMC98921. Replication and Control of Circular Bacterial Plasmids: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/
Identification of cellular objective for elucidating the physiological state of plasmid-bearing Escherichia coli using genome-scale in silico analysis.: http://www.ncbi.nlm.nih.gov/pubmed?cmd=Retrieve&list_uids=19224555
Linear plasmids
http://link.springer.com/chapter/10.1007%2F7171_2007_095
Supplies
pBR322: http://www.kptkomet.no/produkter/no_89/biologi/vnr/779616/
