User:Jarle Pahr/Restriction: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jarle Pahr (talk | contribs) No edit summary |
Jarle Pahr (talk | contribs) No edit summary |
||
| Line 14: | Line 14: | ||
http://www.molecularstation.com/dna/restriction-enzyme-digestion/ | http://www.molecularstation.com/dna/restriction-enzyme-digestion/ | ||
iGEM - restriction digest protocol: http://partsregistry.org/Help:Protocols/Restriction_Digest | |||
Revision as of 10:52, 3 February 2013
Notes on procedures and issues relating to restriction digests:
http://en.wikipedia.org/wiki/Restriction_digest
About star activity: https://www.neb.com/tools-and-resources/usage-guidelines/star-activity
Double Digest tool: http://www.thermoscientificbio.com/webtools/doubledigest/
Enzyme volume should not exceed 10 % of total reaction volume, due to glycerol content.
NEB Double Digest finder: https://www.neb.com/tools-and-resources/interactive-tools/double-digest-finder
http://utminers.utep.edu/rwebb/html/restriction_digestion.html
http://www.molecularstation.com/dna/restriction-enzyme-digestion/
iGEM - restriction digest protocol: http://partsregistry.org/Help:Protocols/Restriction_Digest
